Anti-Collagen I + Collagen III抗体(ab24135)
Key features and details
- Rabbit polyclonal to Collagen I + Collagen III
- Suitable for: IHC-Fr, ELISA
- Reacts with: Pig
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-Collagen I + Collagen III抗体
参阅全部 Collagen I + Collagen III 一抗 -
描述
兔多克隆抗体to Collagen I + Collagen III -
宿主
Rabbit -
特异性
Specificity is measured by ELISA using pure pig collagen types I and III. No binding to pig serum proteins is seen at similar dilutions of the antibody. No cross reactivity seen against pig basement membrane collagens type IV and V in immunostaining. -
经测试应用
适用于: IHC-Fr, ELISAmore details -
种属反应性
与反应: Pig -
免疫原
Full length native collagen type I and III from pig skin.
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阳性对照
- Pig kidney and skin. Cultured porcine cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Lyophilized:Reconstitute with 1ml of distilled water and add preservative if required. -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
存储溶液
pH: 8.00
Constituents: 0.16% Sodium phosphate, 0.38% Sodium borate, 0.87% Sodium chloride, 1% Dextran, 1% Mannitol -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
Pooled antisera are passed over DEAE-cellulose to produce an IgG enriched fraction. Antibodies specific to type I and type III pig collagen are then immobilised by affinity chromatography and eluted with an acidic buffer. The final purification steps are neutralisation, dialysis and lyophilisation. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab24135于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
IHC-Fr |
1/20.
|
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ELISA |
1/3000.
|
说明 |
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IHC-Fr
1/20. |
ELISA
1/3000. |
靶标
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相关性
Collagen I is a fibrillar collagen found in most connective tissues, and the only component of the collagen found in cartilage. Mutations in this gene are associated with osteogenesis imperfecta, Ehlers Danlos syndrome, and idiopathic osteoporosis. Reciprocal translocations between chromosomes 17 and 22, where this gene and the gene for platelet derived growth factor beta are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans, resulting from unregulated expression of the growth factor. Two transcripts, resulting from the use of alternate polyadenylation signals, have been identified for this gene. Collagen III is a fibrillar collagen that is found in extensible connective tissues such as skin, lung, and the vascular system, frequently in association with Collagen I. Mutations in this gene are associated with Ehlers Danlos syndrome type IV, and with aortic and arterial aneurysms. Although alternate transcripts have been detected for this gene, they are the result of mutations; these mutations alter splicing, often leading to the exclusion of multiple exons. -
细胞定位
Secreted -
别名
- Alpha 1 type I collagen antibody
- Alpha 2 type I collagen antibody
- COL1A1 antibody
see all
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab24135 被引用在 1 文献中.
- Luo Q et al. hsa_circ_0001018 promotes papillary thyroid cancer by facilitating cell survival, invasion, G1/S cell cycle progression, and repressing cell apoptosis via crosstalk with miR-338-3p and SOX4. Mol Ther Nucleic Acids 24:591-609 (2021). PubMed: 33898108