概述

  • 产品名称Anti-Collagen I抗体
    参阅全部 Collagen I 一抗
  • 描述
    兔多克隆抗体to Collagen I
  • 特异性Specificity was ascertained by competition ELISA. Complete inhibition was found if the antibody was preincubated with rat collagen type I. Inhibition by rat collagen type III is observed only at 10-20 times higher concentration. No inhibition is found with fibrinectin. fibrogen and laminin.
  • 经测试应用适用于: IHC-Fr, ICC/IF, Dot blot, ELISAmore details
  • 种属反应性
    与反应: Rat
  • 免疫原

    Antibodies to rat collagen type I are raised in rabbits which are numerously immunized with extensively purified native collagen type I extracted from rat tail tendon into dilute acidic buffer after mild pepsin digestion.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液pH: 7.40
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明Pooled antisera are passed over DEAE-cellulose to produce IgG-enriched fraction. Next, the antisera fraction is absorbed with immunobilized rat collagen types III to remove cross-reactive antibodies to antigenic determinants common for this type of collagen. The affinity purified antibody ab24133 is obtained by binding to immobilized native rat collagen type I (the antigen used for immunization), followed by elution with acidic buffer, neutralisation, dialysis, dispensing and lyophilization.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab24133 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-Fr 1/20.
ICC/IF Use at an assay dependent concentration.
Dot blot Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.

靶标

  • 功能Type I collagen is a member of group I collagen (fibrillar forming collagen).
  • 组织特异性Forms the fibrils of tendon, ligaments and bones. In bones the fibrils are mineralized with calcium hydroxyapatite.
  • 疾病相关Defects in COL1A1 are the cause of Caffey disease (CAFFD) [MIM:114000]; also known as infantile cortical hyperostosis. Caffey disease is characterized by an infantile episode of massive subperiosteal new bone formation that typically involves the diaphyses of the long bones, mandible, and clavicles. The involved bones may also appear inflamed, with painful swelling and systemic fever often accompanying the illness. The bone changes usually begin before 5 months of age and resolve before 2 years of age.
    Defects in COL1A1 are a cause of Ehlers-Danlos syndrome type 1 (EDS1) [MIM:130000]; also known as Ehlers-Danlos syndrome gravis. EDS is a connective tissue disorder characterized by hyperextensible skin, atrophic cutaneous scars due to tissue fragility and joint hyperlaxity. EDS1 is the severe form of classic Ehlers-Danlos syndrome.
    Defects in COL1A1 are the cause of Ehlers-Danlos syndrome type 7A (EDS7A) [MIM:130060]; also known as autosomal dominant Ehlers-Danlos syndrome type VII. EDS is a connective tissue disorder characterized by hyperextensible skin, atrophic cutaneous scars due to tissue fragility and joint hyperlaxity. EDS7A is marked by bilateral congenital hip dislocation, hyperlaxity of the joints, and recurrent partial dislocations.
    Defects in COL1A1 are a cause of osteogenesis imperfecta type 1 (OI1) [MIM:166200]. A dominantly inherited connective tissue disorder characterized by bone fragility and blue sclerae. Osteogenesis imperfecta type 1 is non-deforming with normal height or mild short stature, and no dentinogenesis imperfecta.
    Defects in COL1A1 are a cause of osteogenesis imperfecta type 2A (OI2A) [MIM:166210]; also known as osteogenesis imperfecta congenita. A connective tissue disorder characterized by bone fragility, with many perinatal fractures, severe bowing of long bones, undermineralization, and death in the perinatal period due to respiratory insufficiency.
    Defects in COL1A1 are a cause of osteogenesis imperfecta type 3 (OI3) [MIM:259420]. A connective tissue disorder characterized by progressively deforming bones, very short stature, a triangular face, severe scoliosis, grayish sclera, and dentinogenesis imperfecta.
    Defects in COL1A1 are a cause of osteogenesis imperfecta type 4 (OI4) [MIM:166220]; also known as osteogenesis imperfecta with normal sclerae. A connective tissue disorder characterized by moderately short stature, mild to moderate scoliosis, grayish or white sclera and dentinogenesis imperfecta.
    Genetic variations in COL1A1 are a cause of susceptibility to osteoporosis (OSTEOP) [MIM:166710]; also known as involutional or senile osteoporosis or postmenopausal osteoporosis. Osteoporosis is characterized by reduced bone mass, disruption of bone microarchitecture without alteration in the composition of bone. Osteoporotic bones are more at risk of fracture.
    Note=A chromosomal aberration involving COL1A1 is found in dermatofibrosarcoma protuberans. Translocation t(17;22)(q22;q13) with PDGF.
  • 序列相似性Belongs to the fibrillar collagen family.
    Contains 1 fibrillar collagen NC1 domain.
    Contains 1 VWFC domain.
  • 翻译后修饰Proline residues at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains. Proline residues at the second position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some of the chains.
    O-linked glycan consists of a Glc-Gal disaccharide bound to the oxygen atom of a post-translationally added hydroxyl group.
  • 细胞定位Secreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Alpha 1 type I collagen antibody
    • Alpha 2 type I collagen antibody
    • alpha 2 type I procollagen antibody
    • alpha 2(I) procollagen antibody
    • alpha 2(I)-collagen antibody
    • Alpha-1 type I collagen antibody
    • alpha1(I) procollagen antibody
    • CO1A1_HUMAN antibody
    • COL1A1 antibody
    • COL1A2 antibody
    • collagen alpha 1 chain type I antibody
    • Collagen alpha-1(I) chain antibody
    • collagen alpha-1(I) chain preproprotein antibody
    • Collagen I alpha 1 polypeptide antibody
    • Collagen I alpha 2 polypeptide antibody
    • collagen of skin, tendon and bone, alpha-1 chain antibody
    • collagen of skin, tendon and bone, alpha-2 chain antibody
    • Collagen type I alpha 1 antibody
    • Collagen type I alpha 2 antibody
    • EDSC antibody
    • OI1 antibody
    • OI2 antibody
    • OI3 antibody
    • OI4 antibody
    • pro-alpha-1 collagen type 1 antibody
    • type I proalpha 1 antibody
    • type I procollagen alpha 1 chain antibody
    • Type I procollagen antibody
    see all

Anti-Collagen I antibody (ab24133)参考文献

This product has been referenced in:
  • Letouzey V  et al. Tolerance and long-term MRI imaging of gadolinium-modified meshes used in soft organ repair. PLoS One 10:e0120218 (2015). IHC-Fr ; Rat . Read more (PubMed: 25811855) »
  • Serpooshan V  et al. Hydraulic permeability of multilayered collagen gel scaffolds under plastic compression-induced unidirectional fluid flow. Acta Biomater 9:4673-80 (2013). ICC/IF . Read more (PubMed: 22947324) »

See all 4 Publications for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Rat Tissue sections (Rat calvarial sample)
Antigen retrieval step Enzymatic
Specification Rat calvarial sample
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 21°C
Fixative Formaldehyde
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Abcam user community

Verified customer

提交于 Nov 25 2015

Thank you for your calls today and for your questions about this antibody.

The assay sounds similar to an indirect ELISA, with the protocol below:

http://www.abcam.com/index.html?pageconfig=resource&rid=11389

I have em...

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Thank you for contacting us in regards to Anti-Collagen I antibodies to be used in rat samples. As discussed over the phone ab24133 has been used for immunofluorescence and IHC with frozen sections. It has also been tested for specificity with rat ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"