概述

  • 产品名称Anti-Collagen I抗体
    参阅全部 Collagen I 一抗
  • 描述
    兔多克隆抗体to Collagen I
  • 特异性ab23730 reacts with fish collagen type I (tuna fish).
  • 经测试应用适用于: IHC-P, ELISA, RIA, ICC/IFmore details
  • 种属反应性
    与反应: Fish
  • 免疫原

    Purified collagen type I from tuna fish skin.

  • 阳性对照
    • Tuna fish skin.

性能

应用

Our Abpromise guarantee covers the use of ab23730 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use at an assay dependent concentration. Perform enzymatic antigen retrieval before commencing with IHC staining protocol.
ELISA Use at an assay dependent concentration.
RIA Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.

靶标

  • 功能Type I collagen is a member of group I collagen (fibrillar forming collagen).
  • 组织特异性Forms the fibrils of tendon, ligaments and bones. In bones the fibrils are mineralized with calcium hydroxyapatite.
  • 疾病相关Defects in COL1A1 are the cause of Caffey disease (CAFFD) [MIM:114000]; also known as infantile cortical hyperostosis. Caffey disease is characterized by an infantile episode of massive subperiosteal new bone formation that typically involves the diaphyses of the long bones, mandible, and clavicles. The involved bones may also appear inflamed, with painful swelling and systemic fever often accompanying the illness. The bone changes usually begin before 5 months of age and resolve before 2 years of age.
    Defects in COL1A1 are a cause of Ehlers-Danlos syndrome type 1 (EDS1) [MIM:130000]; also known as Ehlers-Danlos syndrome gravis. EDS is a connective tissue disorder characterized by hyperextensible skin, atrophic cutaneous scars due to tissue fragility and joint hyperlaxity. EDS1 is the severe form of classic Ehlers-Danlos syndrome.
    Defects in COL1A1 are the cause of Ehlers-Danlos syndrome type 7A (EDS7A) [MIM:130060]; also known as autosomal dominant Ehlers-Danlos syndrome type VII. EDS is a connective tissue disorder characterized by hyperextensible skin, atrophic cutaneous scars due to tissue fragility and joint hyperlaxity. EDS7A is marked by bilateral congenital hip dislocation, hyperlaxity of the joints, and recurrent partial dislocations.
    Defects in COL1A1 are a cause of osteogenesis imperfecta type 1 (OI1) [MIM:166200]. A dominantly inherited connective tissue disorder characterized by bone fragility and blue sclerae. Osteogenesis imperfecta type 1 is non-deforming with normal height or mild short stature, and no dentinogenesis imperfecta.
    Defects in COL1A1 are a cause of osteogenesis imperfecta type 2A (OI2A) [MIM:166210]; also known as osteogenesis imperfecta congenita. A connective tissue disorder characterized by bone fragility, with many perinatal fractures, severe bowing of long bones, undermineralization, and death in the perinatal period due to respiratory insufficiency.
    Defects in COL1A1 are a cause of osteogenesis imperfecta type 3 (OI3) [MIM:259420]. A connective tissue disorder characterized by progressively deforming bones, very short stature, a triangular face, severe scoliosis, grayish sclera, and dentinogenesis imperfecta.
    Defects in COL1A1 are a cause of osteogenesis imperfecta type 4 (OI4) [MIM:166220]; also known as osteogenesis imperfecta with normal sclerae. A connective tissue disorder characterized by moderately short stature, mild to moderate scoliosis, grayish or white sclera and dentinogenesis imperfecta.
    Genetic variations in COL1A1 are a cause of susceptibility to osteoporosis (OSTEOP) [MIM:166710]; also known as involutional or senile osteoporosis or postmenopausal osteoporosis. Osteoporosis is characterized by reduced bone mass, disruption of bone microarchitecture without alteration in the composition of bone. Osteoporotic bones are more at risk of fracture.
    Note=A chromosomal aberration involving COL1A1 is found in dermatofibrosarcoma protuberans. Translocation t(17;22)(q22;q13) with PDGF.
  • 序列相似性Belongs to the fibrillar collagen family.
    Contains 1 fibrillar collagen NC1 domain.
    Contains 1 VWFC domain.
  • 翻译后修饰Proline residues at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains. Proline residues at the second position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some of the chains.
    O-linked glycan consists of a Glc-Gal disaccharide bound to the oxygen atom of a post-translationally added hydroxyl group.
  • 细胞定位Secreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • 别名
    • Alpha 1 type I collagen antibody
    • Alpha 2 type I collagen antibody
    • alpha 2 type I procollagen antibody
    • alpha 2(I) procollagen antibody
    • alpha 2(I)-collagen antibody
    • Alpha-1 type I collagen antibody
    • alpha1(I) procollagen antibody
    • CO1A1_HUMAN antibody
    • COL1A1 antibody
    • COL1A2 antibody
    • collagen alpha 1 chain type I antibody
    • Collagen alpha-1(I) chain antibody
    • collagen alpha-1(I) chain preproprotein antibody
    • Collagen I alpha 1 polypeptide antibody
    • Collagen I alpha 2 polypeptide antibody
    • collagen of skin, tendon and bone, alpha-1 chain antibody
    • collagen of skin, tendon and bone, alpha-2 chain antibody
    • Collagen type I alpha 1 antibody
    • Collagen type I alpha 2 antibody
    • EDSC antibody
    • OI1 antibody
    • OI2 antibody
    • OI3 antibody
    • OI4 antibody
    • pro-alpha-1 collagen type 1 antibody
    • type I proalpha 1 antibody
    • type I procollagen alpha 1 chain antibody
    • Type I procollagen antibody
    see all

Anti-Collagen I antibody 图像

  • ab23730 staining Collagen I in wholemount Zebrafish cells by Immunocytochemistry/ Immunofluorescence. The cells were paraformaldehyde fixed, permeabilised in acetone at 4°C for 6 minutes and then blocked using 2% sheep serum in PBDT for 1 hour at 24°C. Samples were then incubated with primary antibody at 1/40 for 18 hours. The secondary antibody used was a goat anti-rabbit IgG (H+L) conjugated to Alexa Fluor® 488 (green) used at a 1/800 dilution.
    Note staining of epidermis and actinotrichia.

    See Abreview

Anti-Collagen I antibody (ab23730)参考文献

This product has been referenced in:
  • Richardson R  et al. Adult zebrafish as a model system for cutaneous wound-healing research. J Invest Dermatol 133:1655-65 (2013). Read more (PubMed: 23325040) »

See 1 Publication for this product

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Application Immunocytochemistry/ Immunofluorescence
Sample Zebrafish Cell (wholemount)
Specification wholemount
Fixative Paraformaldehyde
Permeabilization Yes - acetone at 4C for 6 min
Blocking step sheep serum in PBDT as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 24°C
Username

Mrs. Asha Rani

Verified customer

提交于 May 21 2010

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Fish Tissue sections (Skin and bone gelatin)
Specification Skin and bone gelatin
Fixative Paraformaldehyde
Permeabilization Yes - Triton-X 0.2%
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
Username

Abcam user community

Verified customer

提交于 May 26 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"