Anti-Collagen I抗体[5D8-G9] (ab23446)

概述

  • 产品名称
    Anti-Collagen I抗体[5D8-G9]
    参阅全部 Collagen I 一抗
  • 描述
    小鼠单克隆抗体[5D8-G9] to Collagen I
  • 特异性
    Ab23446 is highly specific for type I collagen. The native triple-helical conformation is required for reaction. It has no cross reactivity with type II, III, V and VI collagens. There is no evidence for cross reactivity with other connnective tissue proteins (laminin, fibronectin, elastin).
  • 经测试应用
    适用于: ELISA, ICC/IF, IP, Flow Cyt, WB, IHC-Frmore details
  • 种属反应性
    与反应: Sheep, Goat, Cow, Dog, Human, Pig
    不与反应: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cat, Kangaroo
  • 免疫原

    Full length native protein (purified) corresponding to Human Collagen I.
    Database link: P02452

  • 表位
    The epitope recognised by ab23446 is located near the C terminal of the molecule.
  • 阳性对照
    • human and bovine skin

性能

应用

Our Abpromise guarantee covers the use of ab23446 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/1000.
ICC/IF Use at an assay dependent concentration. PubMed: 20161070
IP Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration. PubMed: 20161070ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
WB Use at an assay dependent concentration. Predicted molecular weight: 134 kDa.

Conditions need to be native to detect Collagen I with this monoclonal antibody, hence would only detect non-denatured, non-reduced collagen type 1.

IHC-Fr Use at an assay dependent concentration. Note: If fixation of tissue is required, acetone or ethanol is recommended.

靶标

  • 功能
    Type I collagen is a member of group I collagen (fibrillar forming collagen).
  • 组织特异性
    Forms the fibrils of tendon, ligaments and bones. In bones the fibrils are mineralized with calcium hydroxyapatite.
  • 疾病相关
    Defects in COL1A1 are the cause of Caffey disease (CAFFD) [MIM:114000]; also known as infantile cortical hyperostosis. Caffey disease is characterized by an infantile episode of massive subperiosteal new bone formation that typically involves the diaphyses of the long bones, mandible, and clavicles. The involved bones may also appear inflamed, with painful swelling and systemic fever often accompanying the illness. The bone changes usually begin before 5 months of age and resolve before 2 years of age.
    Defects in COL1A1 are a cause of Ehlers-Danlos syndrome type 1 (EDS1) [MIM:130000]; also known as Ehlers-Danlos syndrome gravis. EDS is a connective tissue disorder characterized by hyperextensible skin, atrophic cutaneous scars due to tissue fragility and joint hyperlaxity. EDS1 is the severe form of classic Ehlers-Danlos syndrome.
    Defects in COL1A1 are the cause of Ehlers-Danlos syndrome type 7A (EDS7A) [MIM:130060]; also known as autosomal dominant Ehlers-Danlos syndrome type VII. EDS is a connective tissue disorder characterized by hyperextensible skin, atrophic cutaneous scars due to tissue fragility and joint hyperlaxity. EDS7A is marked by bilateral congenital hip dislocation, hyperlaxity of the joints, and recurrent partial dislocations.
    Defects in COL1A1 are a cause of osteogenesis imperfecta type 1 (OI1) [MIM:166200]. A dominantly inherited connective tissue disorder characterized by bone fragility and blue sclerae. Osteogenesis imperfecta type 1 is non-deforming with normal height or mild short stature, and no dentinogenesis imperfecta.
    Defects in COL1A1 are a cause of osteogenesis imperfecta type 2A (OI2A) [MIM:166210]; also known as osteogenesis imperfecta congenita. A connective tissue disorder characterized by bone fragility, with many perinatal fractures, severe bowing of long bones, undermineralization, and death in the perinatal period due to respiratory insufficiency.
    Defects in COL1A1 are a cause of osteogenesis imperfecta type 3 (OI3) [MIM:259420]. A connective tissue disorder characterized by progressively deforming bones, very short stature, a triangular face, severe scoliosis, grayish sclera, and dentinogenesis imperfecta.
    Defects in COL1A1 are a cause of osteogenesis imperfecta type 4 (OI4) [MIM:166220]; also known as osteogenesis imperfecta with normal sclerae. A connective tissue disorder characterized by moderately short stature, mild to moderate scoliosis, grayish or white sclera and dentinogenesis imperfecta.
    Genetic variations in COL1A1 are a cause of susceptibility to osteoporosis (OSTEOP) [MIM:166710]; also known as involutional or senile osteoporosis or postmenopausal osteoporosis. Osteoporosis is characterized by reduced bone mass, disruption of bone microarchitecture without alteration in the composition of bone. Osteoporotic bones are more at risk of fracture.
    Note=A chromosomal aberration involving COL1A1 is found in dermatofibrosarcoma protuberans. Translocation t(17;22)(q22;q13) with PDGF.
  • 序列相似性
    Belongs to the fibrillar collagen family.
    Contains 1 fibrillar collagen NC1 domain.
    Contains 1 VWFC domain.
  • 翻译后修饰
    Proline residues at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains. Proline residues at the second position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some of the chains.
    O-linked glycan consists of a Glc-Gal disaccharide bound to the oxygen atom of a post-translationally added hydroxyl group.
  • 细胞定位
    Secreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Alpha 1 type I collagen antibody
    • Alpha 2 type I collagen antibody
    • alpha 2 type I procollagen antibody
    • alpha 2(I) procollagen antibody
    • alpha 2(I)-collagen antibody
    • Alpha-1 type I collagen antibody
    • alpha1(I) procollagen antibody
    • CO1A1_HUMAN antibody
    • COL1A1 antibody
    • COL1A2 antibody
    • collagen alpha 1 chain type I antibody
    • Collagen alpha-1(I) chain antibody
    • collagen alpha-1(I) chain preproprotein antibody
    • Collagen I alpha 1 polypeptide antibody
    • Collagen I alpha 2 polypeptide antibody
    • collagen of skin, tendon and bone, alpha-1 chain antibody
    • collagen of skin, tendon and bone, alpha-2 chain antibody
    • Collagen type I alpha 1 antibody
    • Collagen type I alpha 2 antibody
    • EDSC antibody
    • OI1 antibody
    • OI2 antibody
    • OI3 antibody
    • OI4 antibody
    • pro-alpha-1 collagen type 1 antibody
    • type I proalpha 1 antibody
    • type I procollagen alpha 1 chain antibody
    • Type I procollagen antibody
    see all

Anti-Collagen I antibody [5D8-G9] 图像

  • Immunohistochemistry (Frozen sections) analysis of sheep vagina tissue labelling Collagen I with ab23446.

  • Immunohistochemistry (Frozen sections) analysis of human endometrium tissue labelling Collagen I with ab23446.

Anti-Collagen I antibody [5D8-G9] (ab23446)参考文献

This product has been referenced in:
  • Simental-Mendía M  et al. Cotransfected human chondrocytes: over-expression of IGF-I and SOX9 enhances the synthesis of cartilage matrix components collagen-II and glycosaminoglycans. Braz J Med Biol Res 48:1063-70 (2015). Read more (PubMed: 26445237) »
  • Jurgens WJ  et al. One-step surgical procedure for the treatment of osteochondral defects with adipose-derived stem cells in a caprine knee defect: a pilot study. Biores Open Access 2:315-25 (2013). IHC ; Goat . Read more (PubMed: 23914338) »

See all 5 Publications for this product

Product Wall

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (human bone marrow derived MSC)
Permeabilization
No
Specification
human bone marrow derived MSC
Blocking step
BSA as blocking agent for 40 minute(s) · Concentration: 10% · Temperature: RT°C
Fixative
Formaldehyde
Username

Nicole Kohli

Verified customer

提交于 Apr 05 2017

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Goat Tissue sections (Bone and Hyaline Cartilage)
Antigen retrieval step
Enzymatic - Buffer/Enzyme Used: protease
Permeabilization
Yes - PBS Tween20
Specification
Bone and Hyaline Cartilage
Fixative
Alcohol, formalin and acid acetic
Username

Abcam user community

Verified customer

提交于 Jul 12 2016

Abreviews
Application
ELISA
Sample
Human Purified protein (Human Collagen Type I)
Specification
Human Collagen Type I
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 37°C
Type
Sandwich (Detection)
Username

Abcam user community

Verified customer

提交于 Jun 12 2008

We recommend the cutting of fixed (using methanol or acetone) 6 um thick frozen sections, incubating with the antibody, washing twice for 10 min in PBS and visualising with sheep anti mouse FITC conjugated antibody. Wash twice again for 10 min in PBS a...

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The native triple-helical conformation is required for reaction.

These two antibodies are not the same clone and come from different immunization experiments. ab90395 has not been tested for it's suitability in flow cytometry. I cannot recommend ab90395 as the antibody does not recognize denatured protein and th...

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