概述

  • 产品名称Anti-COLEC11抗体
    参阅全部 COLEC11 一抗
  • 描述
    兔多克隆抗体to COLEC11
  • 经测试应用适用于: ICC/IF, WB, ELISAmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide selected from the N-terminal region of Human COLEC11 conjugated to KLH (NP_076932).

  • 阳性对照
    • MDA-MB231 cell line lysate

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • 纯度Ammonium Sulphate Precipitation
  • 纯化说明ab91483 is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab91483 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 5 µg/ml.
WB 1/50 - 1/100. Predicted molecular weight: 29 kDa.
ELISA 1/1000.

靶标

  • 功能Lectin that binds to various sugars including fucose and mannose. Has a higher affinity for fucose compared to mannose. Does not bind to glucose, N-acetylglucosamine and N-acetylgalactosamine. Also binds lipopolysaccharides (LPS). Involved in fundamental development serving as a guidance cue for neural crest cell migration.
  • 组织特异性Ubiquitous.
  • 疾病相关Defects in COLEC11 are the cause of 3MC syndrome type 2 (3MC2) [MIM:265050]. 3MC2 is an a disorder characterized by facial dysmorphism that includes hypertelorism, blepharophimosis, blepharoptosis and highly archedDE eyebrows, cleft lip and/or palate, craniosynostosis, learning disability and genital, limb and vesicorenal anomalies. The term 3MC syndrome includes Carnevale, Mingarelli, Malpuech, and Michels syndromes.
  • 序列相似性Belongs to the COLEC10/COLEC11 family.
    Contains 1 C-type lectin domain.
    Contains 1 collagen-like domain.
  • 细胞定位Secreted.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CL K1 antibody
    • CL K1 I antibody
    • CL K1 II antibody
    • CL K1 IIa antibody
    • CL K1 IIb antibody
    • CL-K1 antibody
    • CLK1 antibody
    • COL11_HUMAN antibody
    • COLEC 11 antibody
    • COLEC11 antibody
    • Collectin 11 antibody
    • Collectin kidney I antibody
    • Collectin kidney protein 1 antibody
    • Collectin sub family member 11 antibody
    • Collectin-11 antibody
    • Collectin11 antibody
    • DKFZp686N1868 antibody
    • MGC129470 antibody
    • MGC129471 antibody
    • MGC3279 antibody
    see all

Anti-COLEC11 antibody 图像

  • Anti-COLEC11 antibody (ab91483) at 1/50 dilution + MDA-MB231 cell line lysate at 35 µg

    Predicted band size : 29 kDa
  • ICC/IF image of ab91483 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab91483, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-COLEC11 antibody (ab91483)参考文献

ab91483 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab91483.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"