概述

  • 产品名称
    Anti-COLEC11抗体
  • 描述
    兔多克隆抗体to COLEC11
  • 经测试应用
    适用于: ICC/IF, WB, ELISAmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide selected from the N-terminal region of Human COLEC11 conjugated to KLH (NP_076932).

  • 阳性对照
    • MDA-MB231 cell line lysate

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液
    Preservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • 纯度
    Ammonium Sulphate Precipitation
  • 纯化说明
    ab91483 is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab91483 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 5 µg/ml.
WB 1/50 - 1/100. Predicted molecular weight: 29 kDa.
ELISA 1/1000.

靶标

  • 功能
    Lectin that binds to various sugars including fucose and mannose. Has a higher affinity for fucose compared to mannose. Does not bind to glucose, N-acetylglucosamine and N-acetylgalactosamine. Also binds lipopolysaccharides (LPS). Involved in fundamental development serving as a guidance cue for neural crest cell migration.
  • 组织特异性
    Ubiquitous.
  • 疾病相关
    Defects in COLEC11 are the cause of 3MC syndrome type 2 (3MC2) [MIM:265050]. 3MC2 is an a disorder characterized by facial dysmorphism that includes hypertelorism, blepharophimosis, blepharoptosis and highly archedDE eyebrows, cleft lip and/or palate, craniosynostosis, learning disability and genital, limb and vesicorenal anomalies. The term 3MC syndrome includes Carnevale, Mingarelli, Malpuech, and Michels syndromes.
  • 序列相似性
    Belongs to the COLEC10/COLEC11 family.
    Contains 1 C-type lectin domain.
    Contains 1 collagen-like domain.
  • 细胞定位
    Secreted.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CL K1 antibody
    • CL K1 I antibody
    • CL K1 II antibody
    • CL K1 IIa antibody
    • CL K1 IIb antibody
    • CL-K1 antibody
    • CLK1 antibody
    • COL11_HUMAN antibody
    • COLEC 11 antibody
    • COLEC11 antibody
    • Collectin 11 antibody
    • Collectin kidney I antibody
    • Collectin kidney protein 1 antibody
    • Collectin sub family member 11 antibody
    • Collectin-11 antibody
    • Collectin11 antibody
    • DKFZp686N1868 antibody
    • MGC129470 antibody
    • MGC129471 antibody
    • MGC3279 antibody
    see all

图片

  • Anti-COLEC11 antibody (ab91483) at 1/50 dilution + MDA-MB231 cell line lysate at 35 µg

    Predicted band size : 29 kDa
  • ICC/IF image of ab91483 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab91483, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

文献

ab91483 has not yet been referenced specifically in any publications.

客户评价及客户问答

There are currently no Customer reviews or Questions for ab91483.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

注册