概述

  • 产品名称Anti-COL4A3BP抗体
    参阅全部 COL4A3BP 一抗
  • 描述
    兔多克隆抗体to COL4A3BP
  • 经测试应用适用于: WB, IPmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Opossum, Chinese Hamster, Orangutan, Elephant
  • 免疫原

    Synthetic peptide from a region between residue 1 and 50 of human COL4A3BP (NP_005704.1)

  • 阳性对照
    • Whole cell lysate from Hela and 293T cells.

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应用

Our Abpromise guarantee covers the use of ab72534 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
IP
  • 应用说明IP: Use at 1-4µg/mg of lysate.
    WB: 1/2500 - 1/10000. Detects bands of approximately 70, 71, 110 and 230 kDa (predicted molecular weight: 71 and 70 kDa for isoforms 1 and 2 respectively).

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能May mediate the intracellular trafficking of ceramide in a non-vesicular manner.
    • 组织特异性Widely expressed.
    • 序列相似性Contains 1 PH domain.
      Contains 1 START domain.
    • 结构域The START domain recognizes ceramide and mediates the intermembrane transfer of ceramide.
      The PH domain targets the Golgi apparatus.
      The FFAT motif is required for interaction with VAPA and VAPB.
    • 翻译后修饰Phosphorylation on Ser-132 decreases the affinity toward phosphatidylinositol 4-phosphate at Golgi membranes and reduces ceramide transfer activity.
    • 细胞定位Cytoplasm. Golgi apparatus. Endoplasmic reticulum. Preferentially localized to the Golgi apparatus.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Alpha 3 type IV collagen binding protein antibody
      • C43BP_HUMAN antibody
      • Ceramide transfer protein antibody
      • Ceramide transporter antibody
      • CERT antibody
      • Cert protein antibody
      • CERTL antibody
      • col4a3bp antibody
      • Collagen type IV alpha 3 (Goodpasture antigen) binding protein antibody
      • Collagen type IV alpha 3 binding protein antibody
      • Collagen type IV alpha-3-binding protein antibody
      • FLJ20597 antibody
      • Goodpasture antigen binding protein antibody
      • Goodpasture antigen-binding protein antibody
      • GPBP antibody
      • hCERT antibody
      • Lipid transfer protein CERTL antibody
      • Procollagen type IV alpha 3 (Goodpasture antigen) binding protein antibody
      • StAR related lipid transfer (START) domain containing 11 antibody
      • StAR related lipid transfer protein 11 antibody
      • StAR-related lipid transfer protein 11 antibody
      • StARD 11 antibody
      • StARD11 antibody
      • START domain containing 11 antibody
      • START domain containing protein 11 antibody
      • START domain-containing protein 11 antibody
      see all

    Anti-COL4A3BP antibody 图像

    • All lanes : Anti-COL4A3BP antibody (ab72534) at 0.1 µg/ml

      Lane 1 : Whole cell lysate from Hela cells at 50 µg
      Lane 2 : Whole cell lysate from Hela cells at 15 µg
      Lane 3 : Whole cell lysate from Hela cells at 5 µg
      Lane 4 : Whole cell lysate from 293T cells at 50 µg


      Predicted band size : 71 kDa
      Observed band size : 70,71 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 110 kDa,230 kDa. We are unsure as to the identity of these extra bands.
    • Immunoprecipitation/ Western Blot of COL4A3BP.
      Lane 1: ab72534 at 3µg/mg whole cell lysate.
      Lane 2: Control IgG.
      ab72534 at 1µg/ml for WB.
      Whole cell lysate from Hela cells at 1mg for IP, 20% of IP loaded.
      Chemiluminescence with an exposure time of 30 seconds.

    Anti-COL4A3BP antibody (ab72534)参考文献

    ab72534 has not yet been referenced specifically in any publications.

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