Anti-COL4A3抗体(ab73981)

概述

  • 产品名称Anti-COL4A3抗体
    参阅全部 COL4A3 一抗
  • 描述
    兔多克隆抗体to COL4A3
  • 特异性Reacts specifically with a fragment (26 kDa) and the precursor (162 kDa) of human COL4A3 protein.
  • 经测试应用适用于: WB, IHC-Frmore details
  • 种属反应性
    与反应: Human, Zebrafish
    预测可用于: Mouse, Rat
  • 免疫原

    Synthetic peptide derived from the C-terminal domain of human COL4A3

性能

应用

Our Abpromise guarantee covers the use of ab73981 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/2000. Predicted molecular weight: 26 , 162 kDa.
IHC-Fr 1/50 - 1/100.

靶标

  • 功能Type IV collagen is the major structural component of glomerular basement membranes (GBM), forming a 'chicken-wire' meshwork together with laminins, proteoglycans and entactin/nidogen.
    Tumstatin, a cleavage fragment corresponding to the collagen alpha 3(IV) NC1 domain, possesses both anti-angiogenic and anti-tumor cell activity; these two anti-tumor properties may be regulated via RGD-independent ITGB3-mediated mechanisms.
  • 组织特异性Alpha 3 and alpha 4 type IV collagens are colocalized and present in kidney, eye, basement membranes of lens capsule, cochlea, lung, skeletal muscle, aorta, synaptic fibers, fetal kidney and fetal lung. PubMed:8083201 reports similar levels of expression of alpha 3 and alpha 4 type IV collagens in kidney, but PubMed:7523402 reports that in kidney levels of alpha 3 type IV collagen are significantly lower than those of alpha 4 type IV collagen. According to PubMed:8083201, alpha 3 type IV collagen is not detected in heart, brain, placenta, liver, pancreas, extrasynaptic muscle fibers, endoneurial and perineurial nerves, fetal brain, fetal heart and fetal liver. According to PubMed:7523402, alpha 3 type IV collagen is strongly expressed in pancreas, neuroretina and calvaria and not expressed in adrenal, ileum and skin. Isoform 1 and isoform 3 are strongly expressed in kidney, lung, suprarenal capsule, muscle and spleen, in each of these tissues isoform 1 is more abundant than isoform 3. Isoform 1 and isoform 3 are expressed at low levels in artery, fat, pericardium and peripherical nerve, but not in placenta, mesangium, skin, pleura and cultured umbilical endothelial cells.
  • 疾病相关Note=Autoantibodies against the NC1 domain of alpha 3(IV) are found in Goodpasture syndrome, an autoimmune disease of lung and kidney.
    Defects in COL4A3 are a cause of Alport syndrome autosomal recessive (APSAR) [MIM:203780]. APSAR is characterized by progressive glomerulonephritis, glomerular basement membrane defects, renal failure, sensorineural deafness and specific eye abnormalities (lenticonous and macular flecks). The disorder shows considerable heterogeneity in that families differ in the age of end-stage renal disease and the occurrence of deafness.
    Defects in COL4A3 are a cause of benign familial hematuria (BFH) [MIM:141200]; also known as thin basement membrane nephropathy. BFH is characterized by persistent hematuria, an electron microscopically detectable thin glomerular basement membrane (GBM) and an autosomal dominant mode of inheritance. Renal function remains normal. In children, differentiation between BFH and AS can be difficult, because both disorders are manifested by persistent hematuria and thin GBM at that age.
    Defects in COL4A3 are a cause of Alport syndrome autosomal dominant (APSAD) [MIM:104200]. Alport syndrome is characterized by progressive glomerulonephritis, glomerular basement membrane defects, renal failure, sensorineural deafness and specific eye abnormalities (lenticonous and macular flecks). The disorder shows considerable heterogeneity in that families differ in the age of end-stage renal disease and the occurrence of deafness.
  • 序列相似性Belongs to the type IV collagen family.
    Contains 1 collagen IV NC1 (C-terminal non-collagenous) domain.
  • 结构域Alpha chains of type IV collagen have a non-collagenous domain (NC1) at their C-terminus, frequent interruptions of the G-X-Y repeats in the long central triple-helical domain (which may cause flexibility in the triple helix), and a short N-terminal triple-helical 7S domain.
  • 翻译后修饰Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.
    Isoform 2 contains an additional N-linked glycosylation site.
    Type IV collagens contain numerous cysteine residues which are involved in inter- and intramolecular disulfide bonding. 12 of these, located in the NC1 domain, are conserved in all known type IV collagens.
    The trimeric structure of the NC1 domains is stabilized by covalent bonds between Lys and Met residues.
    Phosphorylated by the Goodpasture antigen-binding protein/COL4A3BP.
  • 细胞定位Secreted > extracellular space > extracellular matrix > basement membrane. Colocalizes with COL4A4 and COL4A5 in GBM, tubular basement membrane (TBM) and synaptic basal lamina (BL).
  • Information by UniProt
  • 数据库链接
  • 别名
    • Alpha 3 type IV collagen antibody
    • Alpha3 type IV collagen antibody
    • CO4A3_HUMAN antibody
    • COL4A 3 antibody
    • Col4a3 antibody
    • Collagen alpha 3(IV) chain antibody
    • Collagen IV alpha 3 polypeptide antibody
    • Collagen type IV alpha 3 (Goodpasture antigen) antibody
    • Collagen type IV alpha 3 antibody
    • Goodpasture antigen antibody
    • OTTHUMP00000195044 antibody
    • Tumstatin antibody
    see all

Anti-COL4A3 antibody (ab73981)参考文献

ab73981 has not yet been referenced specifically in any publications.

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (Mouse Kidney Tissue)
Permeabilization Yes - 0.1M Glycine 6.0M UREA
Specification Mouse Kidney Tissue
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 28°C
Fixative Acetone
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提交于 Oct 20 2015

Regarding the protocol used for COL4A3, the lab were not able to disclose the exact protocol used however, they have informed me that it has been used on human cells in the following paper:

Jones RA, Wang Z, Dookie S, Griffin M. The role of ...

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I have been in contact with the lab who confirmed that ab73981 can recognise a band ˜165 kDa. However, we have no image to communicate because those results are not yet published.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"