Anti-COL22A1抗体(ab121846)

概述

  • 产品名称Anti-COL22A1抗体
  • 描述
    兔多克隆抗体to COL22A1
  • 经测试应用适用于: IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    antigen sequence: LKEELEEIAS EPKSAHVFHV SDFNAIDKIR GKLRRRLCEN VLCPSVRVEG DRFKHTNGGT KEITGFDLMD LFSVKEILGK RENGAQSSYV RMG, corresponding to amino acids 181-273 of Human COL22A1.

  • 阳性对照
    • Human urinary bladder tissue.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

相关产品

应用

Our Abpromise guarantee covers the use of ab121846 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 功能Acts as a cell adhesion ligand for skin epithelial cells and fibroblasts.
  • 组织特异性Restrictive expression is observed at tissue junctions such as the myotendinous junction in skeletal and heart muscle, the articular cartilage-synovial fluid junction, or the border between the anagen hair follicle and the dermis in the skin. It is deposited in the basement membrane zone of the myotendinous junction and the hair follicle and associated with the extrafibrillar matrix in cartilage.
  • 序列相似性Belongs to the fibril-associated collagens with interrupted helices (FACIT) family.
    Contains 16 collagen-like domains.
    Contains 1 TSP N-terminal (TSPN) domain.
    Contains 1 VWFA domain.
  • 细胞定位Secreted > extracellular space > extracellular matrix. Cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 2310067L16Rik antibody
    • C80743 antibody
    • COL22A1 antibody
    • Collagen alpha-1(XXII) chain antibody
    • COMA1_HUMAN antibody
    • Procollagen, type XXII, alpha 1 antibody
    see all

Anti-COL22A1 antibody 图像

  • ab121846 staining COL22A1 in Mouse muscle tendon junction tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde, permeabilized with 0.2% triton X-100 in PBS and blocked with 5% milk for 30 minutes; antigen retrieval was by heat mediation in tris pH 9.0. Samples were incubated with primary antibody (1/100 in PBS) for 16 hours at 4°C. An Alexa Fluor ® 594-conjugated Goat anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody.

    See Abreview

  • ab121846 staining COL22A1 in mouse muscle tendon junction (knee muscle) tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and permeabilized with 0.2% Triton X-100 in PBS; antigen retrieval was by heat mediation in Tris pH 9.0. Samples were incubated with primary antibody (1/100 in PBS) for 16 hours at 4°C. An undiluted biotin-conjugated anti-rabbit IgG polyclonal was used as the secondary antibody.

    See Abreview

  • ab121846 at 1/250 dilution staining COL22A1 in Paraffin-embedded Human urinary bladder tissue by Immunohistochemistry. Note: strong cytoplasmic positivity in urothelial cells.

Anti-COL22A1 antibody (ab121846)参考文献

ab121846 has not yet been referenced specifically in any publications.

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Tris pH 9.0
Sample Mouse Tissue sections (mouse muscle tendon junction (knee muscle))
Specification mouse muscle tendon junction (knee muscle)
Permeabilization Yes - 0.2% Triton X-100 and PBS
Fixative Formaldehyde
Username

Mrs. Maria Cecilia Ybanez

Verified customer

提交于 Jan 23 2014

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5%
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Tris pH 9.0
Sample Mouse Tissue sections (muscle tendon junction)
Specification muscle tendon junction
Permeabilization Yes - 0.2% Triton X-100 and PBS
Fixative Formaldehyde
Username

Dr. Steffen Rickelt

Verified customer

提交于 Nov 04 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"