• 产品名称Anti-COL1A2抗体
    参阅全部 COL1A2 一抗
  • 描述
    兔多克隆抗体to COL1A2
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Zebrafish
  • 免疫原

    Synthetic peptide corresponding to a region within internal amino acids 1044 - 1093 (PGSVGPAGPR GPAGPSGPAG KDGRTGHPGT VGPAGIRGPQ GHQGPAGPPG) of Human COL1A2 (NP_000080)

  • 阳性对照
    • Fetal liver lysate



Our Abpromise guarantee covers the use of ab84713 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 129 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.


  • 功能Type I collagen is a member of group I collagen (fibrillar forming collagen).
  • 组织特异性Forms the fibrils of tendon, ligaments and bones. In bones the fibrils are mineralized with calcium hydroxyapatite.
  • 疾病相关Ehlers-Danlos syndrome 7B
    Osteogenesis imperfecta 1
    Osteogenesis imperfecta 2
    Ehlers-Danlos syndrome, autosomal recessive, cardiac valvular form
    Osteogenesis imperfecta 3
    Osteogenesis imperfecta 4
    A chromosomal aberration involving COL1A2 may be a cause of lipoblastomas, which are benign tumors resulting from transformation of adipocytes, usually diagnosed in children. Translocation t(7;8)(p22;q13) with PLAG1.
  • 序列相似性Belongs to the fibrillar collagen family.
    Contains 1 fibrillar collagen NC1 domain.
  • 结构域The C-terminal propeptide, also known as COLFI domain, have crucial roles in tissue growth and repair by controlling both the intracellular assembly of procollagen molecules and the extracellular assembly of collagen fibrils. It binds a calcium ion which is essential for its function.
  • 翻译后修饰Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.
  • 细胞定位Secreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Alpha 2 collagen type I antibody
    • Alpha 2 type I collagen antibody
    • Alpha 2 type I procollagen antibody
    • Alpha 2(I) collagen antibody
    • Alpha 2(I) procollagen antibody
    • Alpha-2 type I collagen antibody
    • CO1A2_HUMAN antibody
    • COL1A2 antibody
    • Collagen alpha 2(I) chain antibody
    • Collagen alpha-2(I) chain antibody
    • Collagen I alpha 2 polypeptide antibody
    • Collagen of skin tendon and bone alpha 2 chain antibody
    • Collagen type I alpha 2 antibody
    • OI4 antibody
    • Osteogenesis imperfecta type IV antibody
    • Type I procollagen antibody
    see all

Anti-COL1A2 antibody 图像

  • Anti-COL1A2 antibody (ab84713) at 1 µg/ml (in 5% skim milk / PBS buffer) + Human fetal liver lysate at 10 µg

    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 129 kDa
    Observed band size : 144 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 92 kDa. We are unsure as to the identity of these extra bands.

Anti-COL1A2 antibody (ab84713)参考文献

ab84713 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Dog Tissue lysate - whole (kidney, bladder, ureter)
Loading amount 200 µg
Specification kidney, bladder, ureter
Gel Running Conditions Reduced Denaturing (6%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C

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提交于 Mar 09 2011