The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Predicted molecular weight: 129 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
功能Type I collagen is a member of group I collagen (fibrillar forming collagen).
组织特异性Forms the fibrils of tendon, ligaments and bones. In bones the fibrils are mineralized with calcium hydroxyapatite.
疾病相关Ehlers-Danlos syndrome 7B Osteogenesis imperfecta 1 Osteogenesis imperfecta 2 Ehlers-Danlos syndrome, autosomal recessive, cardiac valvular form Osteogenesis imperfecta 3 Osteogenesis imperfecta 4 A chromosomal aberration involving COL1A2 may be a cause of lipoblastomas, which are benign tumors resulting from transformation of adipocytes, usually diagnosed in children. Translocation t(7;8)(p22;q13) with PLAG1.
序列相似性Belongs to the fibrillar collagen family. Contains 1 fibrillar collagen NC1 domain.
结构域The C-terminal propeptide, also known as COLFI domain, have crucial roles in tissue growth and repair by controlling both the intracellular assembly of procollagen molecules and the extracellular assembly of collagen fibrils. It binds a calcium ion which is essential for its function.
翻译后修饰Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.
细胞定位Secreted > extracellular space > extracellular matrix.