The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
功能May act as a cross-bridge between fibrils and other extracellular matrix molecules. Involved in skeletal myogenesis in the developing esophagus. May play a role in organization of the pericellular matrix or the sphinteric smooth muscle.
组织特异性Localized to vascular, neuronal, mesenchymal, and some epithelial basement membrane zones in umbilical cord.
序列相似性Belongs to the fibril-associated collagens with interrupted helices (FACIT) family. Contains 1 TSP N-terminal (TSPN) domain.
结构域The numerous interruptions in the triple helix may make this molecule either elastic or flexible.
翻译后修饰Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.
细胞定位Secreted > extracellular space > extracellular matrix.