概述

  • 产品名称
    Anti-COL11A1抗体
    参阅全部 COL11A1 一抗
  • 描述
    兔多克隆抗体to COL11A1
  • 经测试应用
    适用于: IHC-Fr, ICC/IF, WB, ELISAmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Synthetic peptide from an internal sequence of Human COL11A1.

  • 阳性对照
    • K562 cells.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • 存储溶液
    Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab64883 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-Fr Use at an assay dependent concentration.
ICC/IF 1/50. (see Abreview)
WB 1/500 - 1/1000. Detects a band of approximately >170 kDa (predicted molecular weight: 181 kDa).
ELISA 1/10000.

靶标

  • 功能
    May play an important role in fibrillogenesis by controlling lateral growth of collagen II fibrils.
  • 组织特异性
    Cartilage, placenta and some tumor or virally transformed cell lines. Isoforms using exon IIA or IIB are found in the cartilage while isoforms using only exon IIB are found in the tendon.
  • 疾病相关
    Defects in COL11A1 are the cause of Stickler syndrome type 2 (STL2) [MIM:604841]; also known as Stickler syndrome vitreous type 2. STL2 is an autosomal dominant form of Stickler syndrome, an inherited disorder that associates ocular signs with more or less complete forms of Pierre Robin sequence, bone disorders and sensorineural deafness. Ocular disorders may include juvenile cataract, myopia, strabismus, vitreoretinal or chorioretinal degeneration, retinal detachment, and chronic uveitis. Robin sequence includes an opening in the roof of the mouth (a cleft palate), a large tongue (macroglossia), and a small lower jaw (micrognathia). Bones are affected by slight platyspondylisis and large, often defective epiphyses. Juvenile joint laxity is followed by early signs of arthrosis. The degree of hearing loss varies among affected individuals and may become more severe over time. Syndrome expressivity is variable.
    Defects in COL11A1 are the cause of Marshall syndrome (MARSHS) [MIM:154780]. It is an autosomal dominant disorder with ocular, orofacial, auditory and skeletal manifestations. It shares several features with Stickler syndrome, such as midfacial hypoplasia, high myopia, and sensorineural-hearing deficit.
  • 序列相似性
    Belongs to the fibrillar collagen family.
    Contains 1 fibrillar collagen NC1 domain.
    Contains 1 TSP N-terminal (TSPN) domain.
  • 翻译后修饰
    Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.
  • 细胞定位
    Secreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • 数据库链接
  • 别名
    • COBA1_HUMAN antibody
    • COL11A1 antibody
    • COLL6 antibody
    • Collagen alpha 1 antibody
    • Collagen alpha-1(XI) chain antibody
    • collagen XI alpha 1 antibody
    • collagen XI, alpha 1 polypeptide antibody
    • collagen, type XI, alpha 1 antibody
    • STL2 antibody
    • STL3 antibody
    • XI chain precursor antibody
    see all

图片

  • All lanes : Anti-COL11A1 antibody (ab64883) at 1/500 dilution

    Lane 1 : extracts from K562 cells (5-30µg total protein)
    Lane 2 : extracts from K562 cells (5-30µg total protein) and 5-10µg of the immunising peptide.


    Predicted band size : 181 kDa
    Observed band size : 181 kDa
  • ab64883 staining of frozen human fetal heart tissue sections (IHC-Fr). Sections were fixed with paraformaldehyde and permeabilized by saponin. The primary antibody was diluted 1/200 and incubated with the sample for 1 hour 30 minutes at 37°C. An Alexa Fluor conjugated goat anti-rabbit antibody was used as the secondary.

    This image is courtesy of an anonymous Abreview (April 2009)

  • Immunohistochemical analysis of frozen murine ovary tissue taken from post-natal day 23-29 wild-type (+/+) and ERß-null (-/-) mice.

    COL11A1 was stained with ab64883, at 1/200 dilution.

文献

This product has been referenced in:
  • Lichtenberger BM  et al. Epidermal ß-catenin activation remodels the dermis via paracrine signalling to distinct fibroblast lineages. Nat Commun 7:10537 (2016). IHC ; Mouse . Read more (PubMed: 26837596) »
  • Liu W  et al. Macromolecular organization and fine structure of the human basilar membrane - RELEVANCE for cochlear implantation. Cell Tissue Res 360:245-62 (2015). Read more (PubMed: 25663274) »

See all 6 Publications for this product

客户评价及客户问答

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (tooth)
Antigen retrieval step
None
Permeabilization
No
Specification
tooth
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

提交于 Feb 15 2017

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Lymph node)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate buffer ph=6
Permeabilization
No
Specification
Lymph node
Blocking step
Serum as blocking agent for 15 minute(s) · Concentration: 10% · Temperature: RT°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Jul 11 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (MEF, R1 ES cells, E14 ES cells)
Specification
MEF, R1 ES cells, E14 ES cells
Fixative
Paraformaldehyde
Permeabilization
Yes - Triton
Username

Abcam user community

Verified customer

提交于 Jun 20 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (human fetal heart)
Specification
human fetal heart
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.1% saponin in PBS
Blocking step
Serum as blocking agent for 45 minute(s) · Concentration: 10% · Temperature: 37°C
Username

Abcam user community

Verified customer

提交于 Apr 06 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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