Anti-Coilin抗体[Pdelta] (ab11822)


  • 产品名称Anti-Coilin抗体[Pdelta]
    参阅全部 Coilin 一抗
  • 描述
    小鼠单克隆抗体[Pdelta] to Coilin
  • 特异性Two customers have reported no reactivity with mouse samples (either no reactivity or high non-specific background).
  • 经测试应用适用于: ICC, WB, ICC/IFmore details
  • 种属反应性
    与反应: Human, African green monkey
  • 免疫原

    Recombinant fragment corresponding to Human Coilin (C terminal).

  • 表位Monoclonal Anti-coilin reacts with an epitope located between amino acids 363 and 481 of human coilin.
  • 阳性对照
    • HeLa nuclear extract.



Our Abpromise guarantee covers the use of ab11822 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC Use at an assay dependent dilution.
WB Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 80 kDa.
ICC/IF Use a concentration of 1 µg/ml.


  • 功能Is a component of the nuclear coiled bodies (CBS) which are involved in the function or assembly/disassembly of nucleoplasmic snRNPs. During mitosis, CBS disassemble, coinciding with a mitotic-specific phosphorylation of p80 coilin.
  • 组织特异性Found in all the cell types examined.
  • 序列相似性Belongs to the coilin family.
  • 细胞定位Nucleus. Nuclear coiled body located in the interchromatin space between the nucleolus and the nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CLN 80 antibody
    • CLN80 antibody
    • COIL antibody
    • COIL_HUMAN antibody
    • Coilin antibody
    • Coilin p80 antibody
    • p80 antibody
    • P80 coilin antibody
    see all

Anti-Coilin antibody [Pdelta] 图像

  • ICC/IF image of ab11822 stained MCF-7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab11822, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-Coilin antibody [Pdelta] (ab11822)参考文献

This product has been referenced in:
  • Lin Y  et al. Toxic PR Poly-Dipeptides Encoded by the C9orf72 Repeat Expansion Target LC Domain Polymers. Cell 167:789-802.e12 (2016). Human . Read more (PubMed: 27768897) »
  • Lee JH  et al. Involvement of SRSF11 in cell cycle-specific recruitment of telomerase to telomeres at nuclear speckles. Nucleic Acids Res 43:8435-51 (2015). ICC/IF ; Human . Read more (PubMed: 26286192) »

See all 19 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (H1299)
Specification H1299
Fixative Formaldehyde
Permeabilization Yes - 0.1% Triton X-100
Blocking step Serum as blocking agent for 10 minute(s) · Concentration: 10% · Temperature: 25°C

Abcam user community

Verified customer

提交于 Jan 12 2010

Thank you for your enquiry. We would like to confirm that this antibody has been tested and characterized for Western blot and ICC application. It recognizes human Coilin but it does not cross-react with Mouse However we would like to emphasize ...

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