Anti-Cofilin (phospho S3)抗体(ab12866)

概述

  • 产品名称Anti-Cofilin (phospho S3)抗体
    参阅全部 Cofilin 一抗
  • 描述
    兔多克隆抗体to Cofilin (phospho S3)
  • 经测试应用适用于: ICC/IF, WB, IHC-FoFr, IHC-Pmore details
  • 种属反应性
    与反应: Rat, Dog, Human
    预测可用于: Mouse
  • 免疫原

    Synthetic peptide derived from a region of human cofilin that contains serine 3. The sequence is conserved in mouse and rat.

  • 阳性对照
    • MDCK cells treated with staurosporine; Jurkat cells treated with hydrogen peroxide.

性能

应用

Our Abpromise guarantee covers the use of ab12866 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use at an assay dependent concentration.
WB 1/1000. Detects a band of approximately 20 kDa.
IHC-FoFr Use at an assay dependent concentration. PubMed: 17652593
IHC-P Use at an assay dependent concentration. PubMed: 20797537

靶标

  • 功能Controls reversibly actin polymerization and depolymerization in a pH-sensitive manner. It has the ability to bind G- and F-actin in a 1:1 ratio of cofilin to actin. It is the major component of intranuclear and cytoplasmic actin rods.
  • 组织特异性Widely distributed in various tissues.
  • 序列相似性Belongs to the actin-binding proteins ADF family.
    Contains 1 ADF-H domain.
  • 翻译后修饰Phosphorylated on Ser-3 in resting cells.
  • 细胞定位Nucleus matrix. Cytoplasm > cytoskeleton. Almost completely in nucleus in cells exposed to heat shock or 10% dimethyl sulfoxide.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 18 kDa phosphoprotein antibody
    • CFL 1 antibody
    • CFL antibody
    • CFL1 antibody
    • COF1_HUMAN antibody
    • Cofilin 1 antibody
    • Cofilin 1 non muscle antibody
    • Cofilin antibody
    • Cofilin non muscle isoform antibody
    • Cofilin-1 antibody
    • epididymis secretory protein Li 15 antibody
    • HEL-S-15 antibody
    • non-muscle isoform antibody
    • p18 antibody
    see all

Anti-Cofilin (phospho S3) antibody 图像

  • Peptide Competition and Phosphatase Treatment Lysates prepared from MDCK cells treated with staurosporine (1) or left untreated (2-6) were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. Membranes were either left untreated (1-5) or treated with lambda phosphatase (6), blocked with a 5% BSA-TBST buffer for one hour at room temperature, and incubated with the ab12866 antibody for two hours at room temperature in a 3% BSA-TBST buffer, following prior incubation with: no peptide (1, 2, 6), the non phosphopeptide corresponding to the immunogen (3), a generic phosphoserine-containing peptide (4) or, the phosphopeptide immunogen (5). After washing, membranes were incubated with goat F(ab)2 anti-rabbit IgG HRP conjugate and bands were detected using the Pierce SuperSignal method. The data show that only the peptide corresponding to cofilin [pS3] blocks the antibody signal. The data also show that phosphatase stripping eliminates the signal, verifying that the antibody is phospho-specific.
  • ICC/IF image of ab12866 stained MCF7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab12866, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-Cofilin (phospho S3) antibody (ab12866)参考文献

This product has been referenced in:
  • Vajda F  et al. Cell type-specific Nogo-A gene ablation promotes axonal regeneration in the injured adult optic nerve. Cell Death Differ 22:323-35 (2015). Mouse . Read more (PubMed: 25257170) »
  • Speranza L  et al. Activation of 5-HT7 receptor stimulates neurite elongation through mTOR, Cdc42 and actin filaments dynamics. Front Behav Neurosci 9:62 (2015). WB ; Mouse . Read more (PubMed: 25814944) »

See all 22 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (Primary cortical neuronal cells)
Permeabilization Yes - 0.2% Triton X-100
Specification Primary cortical neuronal cells
Blocking step casein solution + Tween 20 as blocking agent for 20 minute(s) · Concentration: 0.2% · Temperature: 25°C
Fixative Ethanol
Username

Abcam user community

Verified customer

提交于 Aug 18 2016

Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (Nervous Tissue (Nucleus Accumbens))
Gel Running Conditions Reduced Denaturing (10% gel)
Loading amount 40 µg
Specification Nervous Tissue (Nucleus Accumbens)
Blocking step Li-Cor Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 50% · Temperature: 21°C
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Mr. Jeffrey Lenz

Verified customer

提交于 Jan 27 2016

Application Western blot
Loading amount 75 µg
Gel Running Conditions Reduced Denaturing (12%)
Sample Mouse Tissue lysate - whole (brain tissue)
Specification brain tissue
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Nov 10 2014

Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing (12)
Sample Human Tissue lysate - whole (Cerebellum, Brain)
Specification Cerebellum, Brain
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Abcam user community

Verified customer

提交于 Apr 02 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Loading amount 50 µg
Gel Running Conditions Reduced Denaturing
Sample Rat Tissue lysate - whole (Brain)
Specification Brain
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
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Abcam user community

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提交于 Jun 24 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Loading amount 50 µg
Gel Running Conditions Reduced Denaturing (12%)
Sample Human Cell lysate - whole cell (Neuroblastoma)
Specification Neuroblastoma
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
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Abcam user community

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提交于 Jun 24 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Neuroblastoma)
Specification Neuroblastoma
Fixative Formaldehyde
Permeabilization Yes - 0.1% Trition X
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 21°C
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提交于 May 13 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Neuroblastoma)
Specification Neuroblastoma
Fixative Paraformaldehyde
Permeabilization Yes - 0.01% Triton X
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

提交于 Sep 04 2012

Thank you very much for your call today and for your questions about ab12866.

I've further discussed the antibody and proposed staining method with my colleague, and even though we have not tested the antibody in exactly this way,your protoco...

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Thank you for your inquiry.
That antibody is our ab12866, rabbit polyclonal to Cofilin (phospho S3).
http://www.abcam.com/Cofilin-phospho-S3-antibody-ab12866.html
We have that reference listed on our publications page.
http://www.ab...

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