Anti-Cofilin抗体[AF2C2] (ab75510)

概述

  • 产品名称Anti-Cofilin抗体[AF2C2]
    参阅全部 Cofilin 一抗
  • 描述
    小鼠单克隆抗体[AF2C2] to Cofilin
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Recombinant full length Cofilin protein (Human) purified from E.coli (His-Cofilin)

  • 阳性对照
    • Jurkat, Raji, SK-N-MC, PC12 and NIH3T3 cell lysates

性能

应用

Our Abpromise guarantee covers the use of ab75510 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent dilution. Detects a band of approximately 18 kDa (predicted molecular weight: 18 kDa).
ELISA 1/2000.

靶标

  • 功能Controls reversibly actin polymerization and depolymerization in a pH-sensitive manner. It has the ability to bind G- and F-actin in a 1:1 ratio of cofilin to actin. It is the major component of intranuclear and cytoplasmic actin rods.
  • 组织特异性Widely distributed in various tissues.
  • 序列相似性Belongs to the actin-binding proteins ADF family.
    Contains 1 ADF-H domain.
  • 翻译后修饰Phosphorylated on Ser-3 in resting cells.
  • 细胞定位Nucleus matrix. Cytoplasm > cytoskeleton. Almost completely in nucleus in cells exposed to heat shock or 10% dimethyl sulfoxide.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 18 kDa phosphoprotein antibody
    • CFL 1 antibody
    • CFL antibody
    • CFL1 antibody
    • COF1_HUMAN antibody
    • Cofilin 1 antibody
    • Cofilin 1 non muscle antibody
    • Cofilin antibody
    • Cofilin non muscle isoform antibody
    • Cofilin-1 antibody
    • epididymis secretory protein Li 15 antibody
    • HEL-S-15 antibody
    • non-muscle isoform antibody
    • p18 antibody
    see all

Anti-Cofilin antibody [AF2C2] 图像

  • All lanes : Anti-Cofilin antibody [AF2C2] (ab75510) at 1/2000 dilution

    Lane 1 : Jurkat cell lysate
    Lane 2 : Raji cell lysate
    Lane 3 : SK-N-MC cell lysate
    Lane 4 : PC12 cell lysate
    Lane 5 : NIH3T3 cell lysate


    Predicted band size : 18 kDa
    Observed band size : 18 kDa

Anti-Cofilin antibody [AF2C2] (ab75510)参考文献

ab75510 has not yet been referenced specifically in any publications.

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The exact epitope that this antibody recognizes has not yet been identified. Since ab75510 is a monoclonal antibody raised against the full length human cofilin protein, the epitope could lie an...

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I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement for one vial of ab75510. To check the status of the order please contact our Customer...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"