概述

  • 产品名称Anti-Cofilin 2抗体
    参阅全部 Cofilin 2 一抗
  • 描述
    山羊多克隆抗体to Cofilin 2
  • 特异性This antibody is expected to recognise both reported isoforms of cofilin 2.
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Sheep, Chicken, Cow, Dog, Pig
  • 免疫原

    Synthetic peptide:

    DIKDRSTLGEK

    , corresponding to C terminal amino acids 142/152 of Human Cofilin 2

  • 阳性对照
    • Human muscle lysate. Lysate prepared from HEK293 cells over-expressing Cofilin 2.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

相关产品

应用

Our Abpromise guarantee covers the use of ab39985 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.01 - 0.03 µg/ml. Detects a band of approximately 18 kDa (predicted molecular weight: 18 kDa).

靶标

  • 功能Controls reversibly actin polymerization and depolymerization in a pH-sensitive manner. It has the ability to bind G- and F-actin in a 1:1 ratio of cofilin to actin. It is the major component of intranuclear and cytoplasmic actin rods.
  • 组织特异性Isoform CFL2b is expressed predominantly in skeletal muscle and heart. Isoform CFL2a is expressed in various tissues.
  • 疾病相关Defects in CFL2 are the cause of nemaline myopathy type 7 (NEM7) [MIM:610687]. A form of nemaline myopathy. Nemaline myopathies are muscular disorders characterized by muscle weakness of varying severity and onset, and abnormal thread-or rod-like structures in muscle fibers on histologic examination. Nemaline myopathy type 7 presents at birth with hypotonia and generalized weakness. Major motor milestones are delayed, but independent ambulation is achieved.
  • 序列相似性Belongs to the actin-binding proteins ADF family.
    Contains 1 ADF-H domain.
  • 翻译后修饰The phosphorylation of Ser-24 may prevent recognition of the nuclear localization signal.
  • 细胞定位Nucleus matrix. Cytoplasm > cytoskeleton.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CFL 2 antibody
    • CFL2 antibody
    • COF2_HUMAN antibody
    • Cofilin 2 muscle antibody
    • Cofilin antibody
    • Cofilin muscle antibody
    • Cofilin muscle isoform antibody
    • Cofilin-2 antibody
    • Cofilin2 antibody
    • muscle isoform antibody
    • NEM 7 antibody
    • NEM7 antibody
    see all

Anti-Cofilin 2 antibody 图像

  • Anti-Cofilin 2 antibody (ab39985) at 0.003 µg/ml (for 1 hour) + Human Muscle lysate in RIPA buffer at 35 µg
    Developed using the ECL technique

    Predicted band size : 18 kDa
    Observed band size : 18 kDa
  • Anti-Cofilin 2 antibody (ab39985) at 0.03 µg/ml + lysate prepared from HEK293 cells over-expressing Cofilin 2

    Predicted band size : 18 kDa
    Observed band size : 23 kDa (why is the actual band size different from the predicted?)

Anti-Cofilin 2 antibody (ab39985)参考文献

ab39985 has not yet been referenced specifically in any publications.

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