Anti-COBLL1抗体(ab64465)

概述

  • 产品名称
    Anti-COBLL1抗体
  • 描述
    兔多克隆抗体to COBLL1
  • 宿主
    Rabbit
  • 经测试应用
    适用于: WB, ELISA, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog
  • 免疫原

    Synthetic peptide corresponding to Human COBLL1 aa 294-343 (N terminal).
    Sequence:

    SAPATPLVNKHRPTFTRSNTISKPYISNTLPSDAPKKRRAPLPPMPASQS

  • 阳性对照
    • HepG2 cell lysate. Kidney tissue.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • 存储溶液
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab64465 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.5 µg/ml. Detects a band of approximately 132 kDa (predicted molecular weight: 132 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

Titre using peptide based assay 1:312500.

IHC-P Use a concentration of 4 - 8 µg/ml.

靶标

图片

  • Anti-COBLL1 antibody (ab64465) at 0.5 µg/ml + HepG2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size: 132 kDa
    Observed band size: 132 kDa
    Additional bands at: 42 kDa. We are unsure as to the identity of these extra bands.

  • ab64465 at 4µg/ml staining COBLL1 in human kidney tissue, epithelial cells of renal tubule (indicated by arrows)
  • ab64465 staining COBLL1 in human bladder cancer tissue by Immunohistochemistry (formaldehyde fixed sections). Tissue was fixed with formaldehyde, a heat mediated antigen retrieval step was performed using citrate buffer. Tissue was blocked with 1% BSA for 30 minutes at room temperature followed by incubation with the primary antibody, at a 1/80 dilution, for 1 hour. A HRP-conjugated goat anti-rabbit polyclonal was used as secondary antibody un-diluted.
    Note that the protein localizes in the cytoplasm.

    See Abreview

文献

ab64465 has not yet been referenced specifically in any publications.

客户评价及客户问答

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (bladder cancer)
Specification
bladder cancer
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate
Permeabilization
No
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: RT°C
Username

Dr. Karin Birkenkamp-Demtroeder

Verified customer

提交于 Apr 29 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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