概述

  • 产品名称
    Anti-Coatomer subunit delta抗体
    参阅全部 Coatomer subunit delta 一抗
  • 描述
    兔多克隆抗体to Coatomer subunit delta
  • 经测试应用
    适用于: WB, ICC/IFmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    Recombinant protein fragment corresponding to a region within amino acids 89 and 492 of Coatomer subunit delta (NP_001646).

  • 阳性对照
    • WB: 293T, A431, H1299, HeLaS3, HepG2 and Raji cell lysates ICC/IF: HeLa cells

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 10% Glycerol, 0.1M Tris, 0.1M Glycine, pH 7
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab96725 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/3000. Predicted molecular weight: 58 kDa.
ICC/IF 1/100 - 1/200.

靶标

  • 功能
    The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins. In mammals, the coatomer can only be recruited by membranes associated to ADP-ribosylation factors (ARFs), which are small GTP-binding proteins; the complex also influences the Golgi structural integrity, as well as the processing, activity, and endocytic recycling of LDL receptors.
  • 组织特异性
    Ubiquitously expressed.
  • 序列相似性
    Belongs to the adaptor complexes medium subunit family. Delta-COP subfamily.
    Contains 1 MHD (mu homology) domain.
  • 细胞定位
    Cytoplasm. Golgi apparatus membrane. Cytoplasmic vesicle > COPI-coated vesicle membrane. The coatomer is cytoplasmic or polymerized on the cytoplasmic side of the Golgi, as well as on the vesicles/buds originating from it.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Archain 1 antibody
    • Archain antibody
    • Archain vesicle transport protein 1 antibody
    • Arcn1 antibody
    • Coatomer delta subunit antibody
    • Coatomer protein complex, subunit delta antibody
    • Coatomer protein delta COP antibody
    • Coatomer subunit delta antibody
    • COPD_HUMAN antibody
    • Delta coat protein antibody
    • Delta COP antibody
    • Delta-coat protein antibody
    • Delta-COP antibody
    see all

图片

  • All lanes : Anti-Coatomer subunit delta antibody (ab96725) at 1/500 dilution

    Lane 1 : H1299 whole cell lysate
    Lane 2 : HepG2 whole cell lysate

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 58 kDa
  • Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells, using ab96725 at 1/200 dilution. Lower image merged with DNA probe.

文献

This product has been referenced in:
  • Sun E  et al. Dissecting the role of COPI complexes in influenza virus infection. J Virol 87:2673-85 (2013). Read more (PubMed: 23255804) »
  • Zhang X  et al. Dopamine receptor D3 regulates endocytic sorting by a Prazosin-sensitive interaction with the coatomer COPI. Proc Natl Acad Sci U S A 109:12485-90 (2012). WB ; Human . Read more (PubMed: 22802617) »

See all 2 Publications for this product

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