Anti-Coatomer subunit delta抗体(ab81651)

概述

  • 产品名称Anti-Coatomer subunit delta抗体
    参阅全部 Coatomer subunit delta 一抗
  • 描述
    兔多克隆抗体to Coatomer subunit delta
  • 经测试应用适用于: WB, ICC/IFmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Zebrafish
  • 免疫原

    Synthetic peptide corresponding to a region within internal ammino acids 289-338 (RDGGLQNMELHGMIMLRISDDKYGRIRLHVENEDKKGVQLQTHPNVDKK L) of human Coatomer subunit delta (NP_001646).

  • 阳性对照
    • HepG2 cell lysate. IF/ICC: HeLa cell line.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab81651 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 58 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ICC/IF Use a concentration of 5 µg/ml.

靶标

  • 功能The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins. In mammals, the coatomer can only be recruited by membranes associated to ADP-ribosylation factors (ARFs), which are small GTP-binding proteins; the complex also influences the Golgi structural integrity, as well as the processing, activity, and endocytic recycling of LDL receptors.
  • 组织特异性Ubiquitously expressed.
  • 序列相似性Belongs to the adaptor complexes medium subunit family. Delta-COP subfamily.
    Contains 1 MHD (mu homology) domain.
  • 细胞定位Cytoplasm. Golgi apparatus membrane. Cytoplasmic vesicle > COPI-coated vesicle membrane. The coatomer is cytoplasmic or polymerized on the cytoplasmic side of the Golgi, as well as on the vesicles/buds originating from it.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Archain 1 antibody
    • Archain antibody
    • Archain vesicle transport protein 1 antibody
    • Arcn1 antibody
    • Coatomer delta subunit antibody
    • Coatomer protein complex, subunit delta antibody
    • Coatomer protein delta COP antibody
    • Coatomer subunit delta antibody
    • COPD_HUMAN antibody
    • Delta coat protein antibody
    • Delta COP antibody
    • Delta-coat protein antibody
    • Delta-COP antibody
    see all

Anti-Coatomer subunit delta antibody 图像

  • ab81651 stained HeLa cells. The cells were 100% methanol fixed for 5 minutes at -20°C and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1hour at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab81651 at 5µg/ml) overnight at +4°C. The secondary antibody (pseudo-colored green) was Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081) used at a 1/1000 dilution for 1hour at room temperature. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1hour at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

  • Anti-Coatomer subunit delta antibody (ab81651) at 1 µg/ml + HepG2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 58 kDa
    Observed band size : 58 kDa

Anti-Coatomer subunit delta antibody (ab81651)参考文献

ab81651 has not yet been referenced specifically in any publications.

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