The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
应用说明WB: Use at an assay dependent dilution. Detects a band of approximately 62 kDa (predicted molecular weight: 62 kDa) in HEK293 cells transiently transfected with CoA synthase
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
功能Bifunctional enzyme that catalyzes the fourth and fifth sequential steps of CoA biosynthetic pathway. The fourth reaction is catalyzed by the phosphopantetheine adenylyltransferase, coded by the coaD domain; the fifth reaction is catalyzed by the dephospho-CoA kinase, coded by the coaE domain. May act as a point of CoA biosynthesis regulation.
组织特异性Expressed in all tissues examined including brain, heart, skeletal muscle, colon, thymus, spleen, kidney, liver, small intestine, placenta, lung and peripheral blood leukocyte. Lowest expression in peripheral blood leukocytes and highest in kidney and liver. Isoform 2 is expressed mainly in the brain.
通路Cofactor biosynthesis; coenzyme A biosynthesis; CoA from (R)-pantothenate: step 4/5. Cofactor biosynthesis; coenzyme A biosynthesis; CoA from (R)-pantothenate: step 5/5.
序列相似性In the central section; belongs to the eukaryotic coaD family. Contains 1 DPCK (dephospho-CoA kinase) domain.
Phosphopantetheine adenylyltransferase / dephosphocoenzyme A kinase antibody
pOV 2 antibody
Anti-COASY (phospho S182) antibody 图像
Western blot - COASY (phospho S182) antibody (ab45994)
Predicted band size : 62 kDa
Western blot analysis of total cell extracts from HEK293 cells transiently transfected with wt and S182A mutant of CoA synthase (CoAsy). This shows that ab45994 specifically recognize CoA synthase phosphorylated at S182.
The bottom panel shows the positive loading control b-actin
Anti-COASY (phospho S182) antibody (ab45994)参考文献
has not yet been referenced specifically in any publications.