Anti-COASY (phospho S182)抗体(ab45994)

概述

  • 产品名称Anti-COASY (phospho S182)抗体
    参阅全部 COASY 一抗
  • 描述
    兔多克隆抗体to COASY (phospho S182)
  • 特异性This antibody is specific for phospho S182 COASY
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 150 - 250 of Human COASY, phosphorylated at S182.

    (Peptide available as ab46197.)

  • 阳性对照
    • HEK293 cells transiently transfected with wt CoA synthase (COASY)

性能

应用

Our Abpromise guarantee covers the use of ab45994 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
  • 应用说明WB: Use at an assay dependent dilution. Detects a band of approximately 62 kDa (predicted molecular weight: 62 kDa) in HEK293 cells transiently transfected with CoA synthase

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Bifunctional enzyme that catalyzes the fourth and fifth sequential steps of CoA biosynthetic pathway. The fourth reaction is catalyzed by the phosphopantetheine adenylyltransferase, coded by the coaD domain; the fifth reaction is catalyzed by the dephospho-CoA kinase, coded by the coaE domain. May act as a point of CoA biosynthesis regulation.
    • 组织特异性Expressed in all tissues examined including brain, heart, skeletal muscle, colon, thymus, spleen, kidney, liver, small intestine, placenta, lung and peripheral blood leukocyte. Lowest expression in peripheral blood leukocytes and highest in kidney and liver. Isoform 2 is expressed mainly in the brain.
    • 通路Cofactor biosynthesis; coenzyme A biosynthesis; CoA from (R)-pantothenate: step 4/5.
      Cofactor biosynthesis; coenzyme A biosynthesis; CoA from (R)-pantothenate: step 5/5.
    • 序列相似性In the central section; belongs to the eukaryotic coaD family.
      Contains 1 DPCK (dephospho-CoA kinase) domain.
    • 细胞定位Cytoplasm.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Bifunctional coenzyme A synthase antibody
      • Bifunctional phosphopantetheine adenylyl transferase/dephospho CoA kinase antibody
      • CoA synthase antibody
      • COASY antibody
      • COASY_HUMAN antibody
      • Coenzyme A synthase antibody
      • Dephospho-CoA kinase antibody
      • Dephospho-CoA pyrophosphorylase antibody
      • Dephosphocoenzyme A kinase antibody
      • DPCK antibody
      • DPCOAK antibody
      • NBP antibody
      • Nucleotide binding protein antibody
      • Pantetheine-phosphate adenylyltransferase antibody
      • Phosphopantetheine adenylyltransferase / dephosphocoenzyme A kinase antibody
      • pOV 2 antibody
      • POV-2 antibody
      • POV2 antibody
      • PPAT antibody
      • UKR1 antibody
      see all

    Anti-COASY (phospho S182) antibody 图像



    • Predicted band size : 62 kDa


      Western blot analysis of total cell extracts from HEK293 cells transiently transfected with wt and S182A mutant of CoA synthase (CoAsy). This shows that ab45994 specifically recognize CoA synthase phosphorylated at S182. The bottom panel shows the positive loading control b-actin

    Anti-COASY (phospho S182) antibody (ab45994)参考文献

    ab45994 has not yet been referenced specifically in any publications.

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