The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use at an assay dependent dilution. Detects a band of approximately 62 kDa (predicted molecular weight: 62 kDa) in HEK293 cells transiently transfected with CoA synthase
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Bifunctional enzyme that catalyzes the fourth and fifth sequential steps of CoA biosynthetic pathway. The fourth reaction is catalyzed by the phosphopantetheine adenylyltransferase, coded by the coaD domain; the fifth reaction is catalyzed by the dephospho-CoA kinase, coded by the coaE domain. May act as a point of CoA biosynthesis regulation.
Expressed in all tissues examined including brain, heart, skeletal muscle, colon, thymus, spleen, kidney, liver, small intestine, placenta, lung and peripheral blood leukocyte. Lowest expression in peripheral blood leukocytes and highest in kidney and liver. Isoform 2 is expressed mainly in the brain.
Cofactor biosynthesis; coenzyme A biosynthesis; CoA from (R)-pantothenate: step 4/5. Cofactor biosynthesis; coenzyme A biosynthesis; CoA from (R)-pantothenate: step 5/5.
In the central section; belongs to the eukaryotic coaD family. Contains 1 DPCK (dephospho-CoA kinase) domain.
Phosphopantetheine adenylyltransferase / dephosphocoenzyme A kinase antibody
pOV 2 antibody
Western blot - COASY (phospho S182) antibody (ab45994)
Predicted band size : 62 kDa
Western blot analysis of total cell extracts from HEK293 cells transiently transfected with wt and S182A mutant of CoA synthase (CoAsy). This shows that ab45994 specifically recognize CoA synthase phosphorylated at S182.
The bottom panel shows the positive loading control b-actin