概述

  • 产品名称Anti-CO4A2抗体
    参阅全部 CO4A2 一抗
  • 描述
    兔多克隆抗体to CO4A2
  • 经测试应用适用于: WB, ELISA, IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse
  • 免疫原

    Synthetic peptide derived from an internal region of Human CO4A2.

  • 阳性对照
    • HeLa cells; colon carcinoma tissue; extracts from Jurkat cells.

性能

应用

Our Abpromise guarantee covers the use of ab69782 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Detects a band of approximately 155 kDa (predicted molecular weight: 168 kDa).
ELISA 1/20000.
IHC-P 1/50 - 1/100.
ICC/IF 1/500 - 1/1000.

靶标

  • 功能Type IV collagen is the major structural component of glomerular basement membranes (GBM), forming a 'chicken-wire' meshwork together with laminins, proteoglycans and entactin/nidogen.
    Canstatin, a cleavage product corresponding to the collagen alpha 2(IV) NC1 domain, possesses both anti-angiogenic and anti-tumor cell activity. It inhibits proliferation and migration of endothelial cells, reduces mitochondrial membrane potential, and induces apoptosis. Specifically induces Fas-dependent apoptosis and activates procaspase-8 and -9 activity. Ligand for alphavbeta3 and alphavbeta5 integrins.
  • 序列相似性Belongs to the type IV collagen family.
    Contains 1 collagen IV NC1 (C-terminal non-collagenous) domain.
  • 结构域Alpha chains of type IV collagen have a non-collagenous domain (NC1) at their C-terminus, frequent interruptions of the G-X-Y repeats in the long central triple-helical domain (which may cause flexibility in the triple helix), and a short N-terminal triple-helical 7S domain.
  • 翻译后修饰Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.
    Type IV collagens contain numerous cysteine residues which are involved in inter- and intramolecular disulfide bonding. 12 of these, located in the NC1 domain, are conserved in all known type IV collagens.
    The trimeric structure of the NC1 domains is stabilized by covalent bonds between Lys and Met residues.
    Proteolytic processing produces the C-terminal NC1 peptide, canstatin.
  • 细胞定位Secreted > extracellular space > extracellular matrix > basement membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Canstatin antibody
    • CO4A2_HUMAN antibody
    • COL4A 2 antibody
    • Col4a2 antibody
    • Collagen alpha 2(IV) chain antibody
    • Collagen, type IV, alpha 2 antibody
    see all

Anti-CO4A2 antibody 图像

  • Left: HeLa cells stained with ab69782 at 1/500 dilution; Right: the antibody was pre-incubated with the immunizing peptide.
  • Left: Human colon carcinoma tissue stained with ab69782 at 1/50 dilution. Right: the antibody was pre-incubated with the immunizing peptide.
  • All lanes : Anti-CO4A2 antibody (ab69782) at 1/500 dilution

    Lane 1 : extracts from Jurkat cells
    Lane 2 : extracts from Jurkat cells with immunizing peptide at 10 µg

    Lysates/proteins at 15 µg per lane.


    Predicted band size : 168 kDa
    Observed band size : 155 kDa (why is the actual band size different from the predicted?)

Anti-CO4A2 antibody (ab69782)参考文献

ab69782 has not yet been referenced specifically in any publications.

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