The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA: This antibody has been used as a coating antibody at 10 ug/ml in a two site ELISA with PS2-B. Typically the ELISA has a working range of 0-20000 pg/ml and a sensitivity of 10 pg/ml when used with biotin conjugated CNTF antibody. Recommended sample volumes are 25-200 µl. Recovery of hCNTF in human plasma is typically > 85 in the ELISA.
IP: This antibody has been used to immunoprecipitate >90% hCNTF in solution using a double-antibody method.
WB: Use at a concentration of 1 - 5 µg/ml. Predicted molecular weight: 24 kDa.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
CNTF is a survival factor for various neuronal cell types. Seems to prevent the degeneration of motor axons after axotomy.