概述

  • 产品名称Anti-CNTF抗体
    参阅全部 CNTF 一抗
  • 描述
    兔多克隆抗体to CNTF
  • 经测试应用适用于: ICC/IF, WB, ELISAmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Recombinant human CNTF.

性能

应用

Our Abpromise guarantee covers the use of ab46172 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/1000.
WB 1/3000. Predicted molecular weight: 24 kDa.
ELISA 1/5000.

靶标

Anti-CNTF antibody 图像

  • ICC/IF image of ab46172 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab46172, 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-CNTF antibody (ab46172)参考文献

This product has been referenced in:
  • Joly S  et al. New mouse retinal stroke model reveals direction-selective circuit damage linked to permanent optokinetic response loss. Invest Ophthalmol Vis Sci 55:4476-89 (2014). Read more (PubMed: 24970264) »
  • Paintlia MK  et al. S-nitrosoglutathione induces ciliary neurotrophic factor expression in astrocytes, which has implications to protect the central nervous system under pathological conditions. J Biol Chem 288:3831-43 (2013). ICC/IF ; Rat . Read more (PubMed: 23264628) »

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"