ab18527 recognised both isoforms of CNPase.
Synthetic peptide conjugated to KLH derived from within residues 200 - 300 of Human CNPase.
(Peptide available as
This antibody gave a positive signal when tested against Human Brain Tissue.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
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Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Use a concentration of 1 µg/ml. Detects a band of approximately 46, 48 kDa (predicted molecular weight: 46, 48 kDa).
Belongs to the cyclic nucleotide phosphodiesterase family.
Membrane. Melanosome. Firmly bound to membrane structures of brain white matter. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
Information by UniProt
2'3' cyclic nucleotide 3' phosphodiesterase antibody
3''-cyclic-nucleotide 3''-phosphodiesterase antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CNPase antibody (ab18527) This image is courtesy of an abreview submitted by Carl Hobbs, King's College London, United Kingdom
IHC-P image of CNPase staining on human brain section using ab18527 (1:2000). The paraffin embedded section was subjected to heat mediated antigen retrieval using citric acid. The sections were then blocked using 1% BSA at 21°C for 10 min. ab18527 was incubated at 21°C for 16 hours. The secondary antibody was goat polyclonal anti-rabbit IgG conjugated to biotin (1:200)
has not yet been referenced specifically in any publications.
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