Anti-CNPase抗体(ab18527)

概述

  • 产品名称Anti-CNPase抗体
    参阅全部 CNPase 一抗
  • 描述
    兔多克隆抗体to CNPase
  • 特异性ab18527 recognised both isoforms of CNPase.
  • 经测试应用适用于: IHC-P, WBmore details
  • 种属反应性
    与反应: Dog, Human
    预测可用于: Cow
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 200 - 300 of Human CNPase.

    (Peptide available as ab19423.)

  • 阳性对照
    • This antibody gave a positive signal when tested against Human Brain Tissue.

性能

应用

Our Abpromise guarantee covers the use of ab18527 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 46, 48 kDa (predicted molecular weight: 46, 48 kDa).

靶标

Anti-CNPase antibody 图像

  • IHC-P image of CNPase staining on human brain section using ab18527 (1:2000). The paraffin embedded section was subjected to heat mediated antigen retrieval using citric acid. The sections were then blocked using 1% BSA at 21°C for 10 min. ab18527 was incubated at 21°C for 16 hours. The secondary antibody was goat polyclonal anti-rabbit IgG conjugated to biotin (1:200)

    See Abreview

Anti-CNPase antibody (ab18527)参考文献

ab18527 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Dog Tissue sections (Cerebellum)
Specification Cerebellum
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization No
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Username

Mr. Carl Hobbs

Verified customer

提交于 Jun 14 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Brain)
Specification Brain
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization No
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Username

Mr. Carl Hobbs

Verified customer

提交于 Jun 14 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"