参阅全部 CNOT2 一抗
WB, ELISAmore details
Synthetic non-phosphopeptide derived from human CNOT2 around the phosphorylation site of serine 101 (S-L-SP-Q-G).
- HepG2 cell extracts, starved (24hours).
- 存放说明Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
- 存储溶液Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
Concentration information loading...
- 纯度Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||1/500 - 1/1000. Detects a band of approximately 48 kDa (predicted molecular weight: 40 kDa).
- 功能The CCR4-NOT complex functions as general transcription regulation complex.
- 组织特异性Ubiquitous. Highly expressed in brain, heart, thymus, spleen, kidney, liver, small intestine, placenta, lung and peripheral blood leukocytes.
- 序列相似性Belongs to the CNOT2/3/5 family.
- 翻译后修饰Phosphorylated upon DNA damage, probably by ATM or ATR.
- 细胞定位Cytoplasm. Nucleus.
Information by UniProt
- CCR4 associated factor 2 antibody
- CCR4 NOT transcription complex subunit 2 antibody
- CCR4-associated factor 2 antibody
Anti-CNOT2 antibody 图像
Western blot - CNOT2 antibody (ab79368)
All lanes : Anti-CNOT2 antibody (ab79368) at 1/500 dilution
Lane 1 : HepG2 cell extract, starved (24hours)
Lane 2 : HepG2 cell extract, starved (24hours) with immunising peptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size : 40 kDa
Observed band size : 48 kDa (why is the actual band size different from the predicted?)
Additional bands at : 110 kDa,117 kDa. We are unsure as to the identity of these extra bands.
Anti-CNOT2 antibody (ab79368)参考文献
has not yet been referenced specifically in any publications.
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