Anti-CNKR2抗体(ab64844)

概述

  • 产品名称Anti-CNKR2抗体
    参阅全部 CNKR2 一抗
  • 描述
    兔多克隆抗体to CNKR2
  • 特异性This antibody detects endogenous levels of total CNKR2 protein.
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    Synthetic peptide (Human) from an internal region of CNKR2.

  • 阳性对照
    • Extracts from Jurkat cells.

性能

应用

Our Abpromise guarantee covers the use of ab64844 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Detects a band of approximately 117 kDa (predicted molecular weight: 118 kDa).
ELISA 1/20000.

靶标

Anti-CNKR2 antibody 图像

  • All lanes : Anti-CNKR2 antibody (ab64844) at 1/500 dilution

    Lane 1 : Extracts from Jurkat cells
    Lane 2 : Extracts from Jurkat cells with blocking peptide at 5 µg

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 118 kDa
    Observed band size : 117 kDa (why is the actual band size different from the predicted?)

    Secondary antibody - anti-rabbit HRP (ab6721)

Anti-CNKR2 antibody (ab64844)参考文献

ab64844 has not yet been referenced specifically in any publications.

Product Wall

Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Rat Tissue lysate - other (brain tissue lysate, cytosolic fraction)
Loading amount 10 µg
Specification brain tissue lysate, cytosolic fraction
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 18°C
Username

Abcam user community

Verified customer

提交于 May 02 2012

Application Western blot
Sample Mouse Tissue lysate - other (Brain tissue lysate, cytosolic fraction)
Loading amount 10 µg
Specification Brain tissue lysate, cytosolic fraction
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 18°C
Username

Dr. Maryna Polyakova

Verified customer

提交于 Apr 13 2012

Thank you for your reply.
Reviewing the remaining antibodies that are already tested in WB and the species you wish to use, the following do not yet have a western blot image, and I would be pleased to arrange a testing discount for these addition...

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Thank you very much for your interest in our antibodies.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"