Anti-CNBP抗体(ab83038)

概述

  • 产品名称Anti-CNBP抗体
    参阅全部 CNBP 一抗
  • 描述
    兔多克隆抗体to CNBP
  • 经测试应用适用于: ELISA, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Guinea pig, Cat, Dog
  • 免疫原

    Synthetic peptide corresponding to a region within N terminal amino acids 2-51 (SSNECFKCGR SGHWARECPT GGGRGRGMRS RGRGGFTSDR GFQFVSSSLP) of Human CNBP (NP_003409).

  • 阳性对照
    • Human fetal brain lysate.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab83038 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA Use at an assay dependent concentration.

Titre using peptide based assay: 1.777777778.

WB Use a concentration of 1 µg/ml. Predicted molecular weight: 19 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

  • 功能Single stranded DNA-binding protein, with specificity to the sterol regulatory element (SRE). Involved in sterol-mediated repression.
  • 组织特异性Present in all tissues examined.
  • 疾病相关Defects in CNBP are the cause of dystrophia myotonica type 2 (DM2) [MIM:602668]; also known as proximal myotonic myopathy (PROMM). A multisystem disease characterized by the association of proximal muscle weakness with myotonia, cardiac manifestations and cataract. Additional features can include hyperhidrosis, testicular atrophy, insulin resistance and diabetes and central nervous system anomalies in rare cases. Note=The causative mutation is a CCTG expansion (mean approximately 5000 repeats) located in intron 1 of the CNBP gene.
  • 序列相似性Contains 7 CCHC-type zinc fingers.
  • 细胞定位Cytoplasm. Endoplasmic reticulum.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CCHC type zinc finger nucleic acid binding protein antibody
    • Cellular nucleic acid binding protein antibody
    • Cellular nucleic acid-binding protein antibody
    • CNBP antibody
    • CNBP_HUMAN antibody
    • CNBP1 antibody
    • DM2 antibody
    • Erythroid differentiation related antibody
    • PROMM antibody
    • Proximal myotonic myopathy nucleic acid binding protein antibody
    • RNF163 antibody
    • Sterol regulatory element binding protein antibody
    • ZCCHC22 antibody
    • Zinc finger protein 273 antibody
    • Zinc finger protein 9 (a cellular retroviral nucleic acid binding protein) antibody
    • Zinc finger protein 9 antibody
    • ZNF9 antibody
    see all

Anti-CNBP antibody 图像

  • Anti-CNBP antibody (ab83038) at 1 µg/ml (in 5% skim milk / PBS buffer) + Human fetal brain lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 19 kDa
    Observed band size : 21 kDa (why is the actual band size different from the predicted?)

Anti-CNBP antibody (ab83038)参考文献

ab83038 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Mouse Cell lysate - whole cell (bone marrow derived macrophages)
Gel Running Conditions Reduced Denaturing (13%)
Loading amount 500000 cells
Specification bone marrow derived macrophages
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
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提交于 Jan 18 2016

I am very pleased to hear you would like to accept our offer and test ab83038 in ICC/IF. This code will give you 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview for ICC/IF and include this code in...

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Thank you very much for your interest in our anti-CNBP antibodies. To our knowledge, neither ab48027 nor ab83038 have been tested in cell staining (immunocytochemistry, ICC/IF). The information about localization in the ER and cytoplasm comes f...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"