• 产品名称
  • 描述
    兔多克隆抗体to CNAP1
  • 经测试应用
    适用于: IHC-P, WBmore details
    不适用于: ICC/IF
  • 种属反应性
    与反应: Human
    预测可用于: Mouse
  • 免疫原

    Synthetic peptide derived from within residues 1350 to the C-terminus of Human CNAP1.

    (Peptide available as ab34337.)

  • 阳性对照
    • HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate, Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate, A431 (Human epithelial carcinoma cell line) Whole Cell Lysate.



Our Abpromise guarantee covers the use of ab34338 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 150 kDa (predicted molecular weight: 157 kDa).
  • 应用说明
    Is unsuitable for ICC/IF.
  • 靶标

    • 相关性
      CNAP1/hCAP-D2 is a regulatory subunit of the condensin complex, a complex required for conversion of interphase chromatin into mitotic-like condensed chromosomes. The condensin complex probably introduces positive supercoils into relaxed DNA in the presence of type I topoisomerases and converts nicked DNA into positive knotted forms in the presence of type II topoisomerases. It may target the condensin complex to DNA via its C-terminal domain (referenced from Swissprot).
    • 细胞定位
      Nuclear and cytoplasmic.
    • 数据库链接
    • 别名
      • 2810406C15Rik antibody
      • 2810465G24Rik antibody
      • CAP-D2 antibody
      • CAPD2 antibody
      • CEP2 antibody
      • CEP250 antibody
      • Chromosome condensation-related SMC-associated protein 1 antibody
      • Chromosome-associated protein D2 antibody
      • CNAP1 antibody
      • CND1 antibody
      • Condensin antibody
      • Condensin complex subunit 1 antibody
      • eg7 antibody
      • hCAP-D2 antibody
      • hCAPD2 antibody
      • KIAA0159 antibody
      • mKIAA0159 antibody
      • NCAPD2 antibody
      • non-SMC condensin I complex, subunit D2 antibody
      • XCAP D2 homolog antibody
      see all

    Anti-CNAP1 antibody 图像

    • Lane 1 : Marker
      Lanes 2 - 4 : Anti-CNAP1 antibody (ab34338) at 1 µg/ml

      Lane 1 : As above
      Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg
      Lane 3 : Jurkat whole cell lysate (ab7899) at 20 µg
      Lane 4 : A431 whole cell lysate (ab7909) at 20 µg

      Lanes 2 - 4 : IR Dye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/15000 dilution

      Performed under reducing conditions.

      Predicted band size : 157 kDa
      Observed band size : 150 kDa (why is the actual band size different from the predicted?)
    • Image courtesy of Human Protein Atlas ab34338 staining CNAP1 in human testis, showing a predominantly nuclear staining pattern in cells of the seminiferous tubule. Paraffin embedded human testis tissue was incubated with ab34338 (1/100 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6.

      ab34338 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues. Further results for this antibody can be found at

    Anti-CNAP1 antibody (ab34338)参考文献

    ab34338 has not yet been referenced specifically in any publications.

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    Thank you for your enquiry regarding ab4448 and for taking the time to provide some useful details of the experiments. I am very sorry to hear that you are having problems with this antibody. Ab4448 is a very high characterized antibody which has b...

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    Abcam has not validated the combination of species/application used in this Abreview.
    Immunocytochemistry/ Immunofluorescence
    Human Cell (HeLa)

    Dr. Kirk Mcmanus

    Verified customer

    提交于 Mar 05 2007