Anti-CNAP1抗体(ab34307)

概述

  • 产品名称
  • 描述
    兔多克隆抗体to CNAP1
  • 经测试应用
    适用于: WBmore details
    不适用于: ICC/IF
  • 种属反应性
    与反应: Human
    预测可用于: Mouse
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1200 - 1300 of Human CNAP1.

    (Peptide available as ab34336.)

  • 阳性对照
    • This antibody gave a positive signal in the following whole cell lysates: HeLa; Jurkat.

性能

应用

Our Abpromise guarantee covers the use of ab34307 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
  • 应用说明
    WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 150 kDa (predicted molecular weight: 157 kDa).

    This antibody does not appear to be suitable for ICC/IF (please see abreview).

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 相关性
      CNAP1/hCAP-D2 is a regulatory subunit of the condensin complex, a complex required for conversion of interphase chromatin into mitotic-like condensed chromosomes. The condensin complex probably introduces positive supercoils into relaxed DNA in the presence of type I topoisomerases and converts nicked DNA into positive knotted forms in the presence of type II topoisomerases. It may target the condensin complex to DNA via its C-terminal domain (referenced from Swissprot).
    • 细胞定位
      Nuclear and cytoplasmic.
    • 数据库链接
    • 别名
      • 2810406C15Rik antibody
      • 2810465G24Rik antibody
      • CAP-D2 antibody
      • CAPD2 antibody
      • CEP2 antibody
      • CEP250 antibody
      • Chromosome condensation-related SMC-associated protein 1 antibody
      • Chromosome-associated protein D2 antibody
      • CNAP1 antibody
      • CND1 antibody
      • Condensin antibody
      • Condensin complex subunit 1 antibody
      • eg7 antibody
      • hCAP-D2 antibody
      • hCAPD2 antibody
      • KIAA0159 antibody
      • mKIAA0159 antibody
      • NCAPD2 antibody
      • non-SMC condensin I complex, subunit D2 antibody
      • XCAP D2 homolog antibody
      see all

    图片

    • All lanes : Anti-CNAP1 antibody (ab34307) at 1 µg/ml

      Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg
      Lane 2 : Jurkat whole cell lysate (ab7899) at 20 µg

      Secondary
      IR Dye 680 conjugated Goat-Anti Rabbit IgG (H&L) at 1/15000 dilution

      Performed under reducing conditions.

      Predicted band size : 157 kDa
      Observed band size : 150 kDa (why is the actual band size different from the predicted?)

    实验方案

    文献

    ab34307 has not yet been referenced specifically in any publications.

    客户评价及客户问答

    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HeLa)
    Specification
    HeLa
    Fixative
    Paraformaldehyde
    Username

    Dr. Kirk Mcmanus

    Verified customer

    提交于 Mar 05 2007

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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