Anti-CNAP1抗体(ab34307)

概述

  • 产品名称Anti-CNAP1抗体
    参阅全部 CNAP1 一抗
  • 描述
    兔多克隆抗体to CNAP1
  • 经测试应用适用于: WBmore details
    不适用于: ICC/IF
  • 种属反应性
    与反应: Human
    预测可用于: Mouse
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1200 - 1300 of Human CNAP1.

    (Peptide available as ab34336.)

  • 阳性对照
    • This antibody gave a positive signal in the following whole cell lysates: HeLa; Jurkat.

性能

应用

Our Abpromise guarantee covers the use of ab34307 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
  • 应用说明WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 150 kDa (predicted molecular weight: 157 kDa).

    This antibody does not appear to be suitable for ICC/IF (please see abreview).

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 相关性CNAP1/hCAP-D2 is a regulatory subunit of the condensin complex, a complex required for conversion of interphase chromatin into mitotic-like condensed chromosomes. The condensin complex probably introduces positive supercoils into relaxed DNA in the presence of type I topoisomerases and converts nicked DNA into positive knotted forms in the presence of type II topoisomerases. It may target the condensin complex to DNA via its C-terminal domain (referenced from Swissprot).
    • 细胞定位Nuclear and cytoplasmic.
    • 数据库链接
    • 别名
      • 2810406C15Rik antibody
      • 2810465G24Rik antibody
      • CAP-D2 antibody
      • CAPD2 antibody
      • CEP2 antibody
      • CEP250 antibody
      • Chromosome condensation-related SMC-associated protein 1 antibody
      • Chromosome-associated protein D2 antibody
      • CNAP1 antibody
      • CND1 antibody
      • Condensin antibody
      • Condensin complex subunit 1 antibody
      • eg7 antibody
      • hCAP-D2 antibody
      • hCAPD2 antibody
      • KIAA0159 antibody
      • mKIAA0159 antibody
      • NCAPD2 antibody
      • non-SMC condensin I complex, subunit D2 antibody
      • XCAP D2 homolog antibody
      see all

    Anti-CNAP1 antibody 图像

    • All lanes : Anti-CNAP1 antibody (ab34307) at 1 µg/ml

      Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg
      Lane 2 : Jurkat whole cell lysate (ab7899) at 20 µg

      Secondary
      IR Dye 680 conjugated Goat-Anti Rabbit IgG (H&L) at 1/15000 dilution

      Performed under reducing conditions.

      Predicted band size : 157 kDa
      Observed band size : 150 kDa (why is the actual band size different from the predicted?)

    Anti-CNAP1 antibody (ab34307)参考文献

    ab34307 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam has not validated the combination of species/application used in this Abreview.
    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cell (HeLa)
    Specification HeLa
    Fixative Paraformaldehyde
    Username

    Dr. Kirk McManus

    Verified customer

    提交于 Mar 05 2007

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"