概述

  • 产品名称Anti-CMG1抗体
    参阅全部 CMG1 一抗
  • 描述
    山羊多克隆抗体to CMG1
  • 经测试应用适用于: IHC-P, WB, ICC/IFmore details
  • 种属反应性
    与反应: Rat, Human
  • 免疫原

    Synthetic peptide:

    KTIVDALHSTSGN

    , corresponding to C terminal amino acids 588-600 of Human CMG1

  • 阳性对照
    • Lysates of cell line HEK293. Human liver tissue. Rat cortical neurons.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris saline. pH 7.3
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab38789 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 5 - 10 µg/ml.
WB Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 75 kDa (predicted molecular weight: 69 kDa). The observed molecular weight corresponds to earlier findings in literature (Momeni et al, BMC Neurol. 2006 Dec 13;6:44.; PMID: 17166276).
ICC/IF Use a concentration of 2.5 µg/ml.

靶标

  • 组织特异性Highly expressed in adult and fetal kidney and expressed at lower level in adult heart, placenta, lung, liver and pancreas, and in fetal heart, lung and liver. Little to no expression was detected in adult brain and skeletal muscle or in fetal brain, thymus and spleen.
  • 翻译后修饰Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 细胞定位Cytoplasmic vesicle. Intracellular vesicular compartment.
  • Information by UniProt
  • 数据库链接
  • 别名
    • intraflagellar transport 74, Chlamydomonas, homolog of antibody
    • Capillary morphogenesis gene 1 protein antibody
    • Capillary morphogenesis protein 1 antibody
    • Ccdc2 antibody
    • CMG 1 antibody
    • CMG-1 antibody
    • Coiled-coil domain containing 2 antibody
    • Coiled-coil domain-containing protein 2 antibody
    • IFT74 antibody
    • IFT74_HUMAN antibody
    • intraflagellar transport 74 homolog (Chlamydomonas) antibody
    • intraflagellar transport 74 homolog antibody
    • Intraflagellar transport protein 74 homolog antibody
    see all

Anti-CMG1 antibody 图像

  • Anti-CMG1 antibody (ab38789) at 0.3 µg/ml + 293 lysate (RIPA buffer, 35µg total protein per lane).

    Predicted band size : 69 kDa
    Observed band size : 75 kDa (why is the actual band size different from the predicted?)
  • ab38789 at 5ug/ml staining CMG1 in human liver tissue section by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections). Tissue underwent antigen retrieval in steam with citrate buffer (pH 6.0). The AP-staining procedure was used for detection.

  • ab38789 at 2.5mg/ml staining CMG1 in primary rat cortical neurons by Immunocytochemistry/ Immunofluorecence. The image shows localization of CMG1 in cell body vesicles, along the neuronal processes. Image B is the control.

Anti-CMG1 antibody (ab38789)参考文献

This product has been referenced in:
  • Boldt K  et al. Disruption of intraflagellar protein transport in photoreceptor cilia causes Leber congenital amaurosis in humans and mice. J Clin Invest 121:2169-80 (2011). Read more (PubMed: 21606596) »
  • Momeni P  et al. Analysis of IFT74 as a candidate gene for chromosome 9p-linked ALS-FTD. BMC Neurol 6:44 (2006). Read more (PubMed: 17166276) »

See all 2 Publications for this product

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