Anti-Clusterin抗体[Hs-3] (ab349)

概述

  • 产品名称Anti-Clusterin抗体[Hs-3]
    参阅全部 Clusterin 一抗
  • 描述
    小鼠单克隆抗体[Hs-3] to Clusterin
  • 经测试应用适用于: WB, ICC/IF, ELISA, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse不与反应: Cow, Cat, Dog
  • 免疫原

    Recombinant full length protein corresponding to Human Clusterin .

性能

应用

Our Abpromise guarantee covers the use of ab349 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent concentration. Predicted molecular weight: 60 kDa.
ICC/IF Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.
IHC-P 1/100.

靶标

  • 功能Isoform 1 functions as extracellular chaperone that prevents aggregation of nonnative proteins. Prevents stress-induced aggregation of blood plasma proteins. Inhibits formation of amyloid fibrils by APP, APOC2, B2M, CALCA, CSN3, SNCA and aggregation-prone LYZ variants (in vitro). Does not require ATP. Maintains partially unfolded proteins in a state appropriate for subsequent refolding by other chaperones, such as HSPA8/HSC70. Does not refold proteins by itself. Binding to cell surface receptors triggers internalization of the chaperone-client complex and subsequent lysosomal or proteasomal degradation. Secreted isoform 1 protects cells against apoptosis and against cytolysis by complement. Intracellular isoforms interact with ubiquitin and SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complexes and promote the ubiquitination and subsequent proteasomal degradation of target proteins. Promotes proteasomal degradation of COMMD1 and IKBKB. Modulates NF-kappa-B transcriptional activity. Nuclear isoforms promote apoptosis. Mitochondrial isoforms suppress BAX-dependent release of cytochrome c into the cytoplasm and inhibit apoptosis. Plays a role in the regulation of cell proliferation.
  • 组织特异性Detected in blood plasma, cerebrospinal fluid, milk, seminal plasma and colon mucosa. Detected in the germinal center of colon lymphoid nodules and in colon parasympathetic ganglia of the Auerbach plexus (at protein level). Ubiquitous. Detected in brain, testis, ovary, liver and pancreas, and at lower levels in kidney, heart, spleen and lung.
  • 序列相似性Belongs to the clusterin family.
  • 翻译后修饰Isoform 1 is proteolytically cleaved on its way through the secretory system, probably within the Golgi lumen.
    Polyubiquitinated, leading to proteasomal degradation.
    Heavily N-glycosylated. About 30% of the protein mass is comprised of complex N-linked carbohydrate.
  • 细胞定位Secreted. Can retrotranslocate from the secretory compartments to the cytosol upon cellular stress and Nucleus. Cytoplasm. Mitochondrion membrane. Cytoplasm, cytosol. Microsome. Endoplasmic reticulum. Cytoplasmic vesicle, secretory vesicle, chromaffin granule. Isoforms lacking the N-terminal signal sequence have been shown to be cytoplasmic and/or nuclear. Secreted isoforms can retrotranslocate from the secretory compartments to the cytosol upon cellular stress. Detected in perinuclear foci that may be aggresomes containing misfolded, ubiquitinated proteins. Detected at the mitochondrion membrane upon induction of apoptosis.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 40 antibody
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Anti-Clusterin antibody [Hs-3] (ab349)参考文献

This product has been referenced in:
  • Thambisetty M  et al. Association of plasma clusterin concentration with severity, pathology, and progression in Alzheimer disease. Arch Gen Psychiatry 67:739-48 (2010). WB . Read more (PubMed: 20603455) »

See 1 Publication for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (testis)
Specification testis
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid pH6 10mM
Permeabilization No
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: rt°C
Username

Mr. Carl Hobbs

Verified customer

提交于 Jun 29 2009

Thank you for your enquiry. The antibody was used for staining of acetone fixed ejaculated human spermatozoa. The antibody did not react on immunoblots with SDS boar, dog, cat and bull sperm extracts, the antibody seems to be human specific. Full info ...

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Thank you for your enquiry. The antibody was used for staining of acetone fixed ejaculated human spermatozoa. The antibody did not react on immunoblots with SDS boar, dog, cat and bull sperm extracts, the antibody seems to be human specific. Full info ...

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Thank you for your enquiry. The antibody was tested only on cells, which were fixed on glass slides. The origioanotor of the product believes that it will be suitable for IHC-Fr, however it has not yet been tested for this application. As a result of y...

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Thank you for your enquiry, I'm really sorry about the delay in my reply, I have had to check with the originator if the antibody was tested in ELISA. I can now tell you that it has not yet been tested so I cannot guarantee that it will work in ELISA. ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"