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RabMAb

Anti-CLPP抗体[EPR7133] (ab124822)

概述

  • 产品名称
    Anti-CLPP抗体[EPR7133]
    参阅全部 CLPP 一抗
  • 描述
    兔单克隆抗体[EPR7133] to CLPP
  • 宿主
    Rabbit
  • 经测试应用
    适用于: IHC-P, WB, IPmore details
    不适用于: Flow Cyt
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    corresponding to Human CLPP.

  • 阳性对照
    • Fetal muscle, Saos-2, A431, 293T, K562, and A549 lysates; K562 cells
  • 常规说明

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

性能

应用

Our Abpromise guarantee covers the use of ab124822 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

WB 1/1000 - 1/10000. Detects a band of approximately 26 kDa (predicted molecular weight: 30 kDa).
IP 1/10 - 1/100.
  • 应用说明
    Is unsuitable for Flow Cyt.
  • 靶标

    • 功能
      Clp cleaves peptides in various proteins in a process that requires ATP hydrolysis. Clp may be responsible for a fairly general and central housekeeping function rather than for the degradation of specific substrates.
    • 组织特异性
      Predominantly expressed in skeletal muscle. Intermediate levels in heart, liver and pancreas. Low in brain, placenta, lung and kidney.
    • 序列相似性
      Belongs to the peptidase S14 family.
    • 细胞定位
      Mitochondrion matrix.
    • Information by UniProt
    • 数据库链接
    • 别名
      • ATP dependent protease ClpAP (E coli) proteolytic subunit antibody
      • ATP dependent protease ClpAP proteolytic subunit antibody
      • ATP dependent protease ClpAP proteolytic subunit human antibody
      • ATP dependent proteolytic subunit homolog (E coli) antibody
      • ATP dependent proteolytic subunit homolog antibody
      • Caseinolytic peptidase ATP dependent proteolytic subunit homolog antibody
      • Caseinolytic protease ATP dependent proteolytic subunit E coli antibody
      • clpP antibody
      • ClpP caseinolytic peptidase ATP dependent proteolytic subunit antibody
      • ClpP caseinolytic peptidase ATP dependent proteolytic subunit homolog antibody
      • CLPP_HUMAN antibody
      • Endopeptidase Clp antibody
      • mitochondrial antibody
      • Putative ATP dependent Clp protease proteolytic subunit mitochondrial antibody
      • Putative ATP dependent Clp protease proteolytic subunit antibody
      • Putative ATP-dependent Clp protease proteolytic subunit antibody
      see all

    图片

    • ab124822 (purified) at 1:20 dilution (2µg) immunoprecipitating CLPP in K562 whole cell lysate.
      Lane 1 (input): K562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysate 10µg
      Lane 2 (+): ab124822 & K562 whole cell lysate
      Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab124822 in K562 whole cell lysate
      For western blotting, VeriBlot for IP secondary antibody (HRP) (ab131366) was used as the secondary antibody at 1:5000 dilution.
      Blocking and diluting buffer: 5% NFDM/TBST.
    • All lanes : Anti-CLPP antibody [EPR7133] (ab124822) at 1/1000 dilution (unpurified)

      Lane 1 : Fetal muscle lysate
      Lane 2 : Saos-2 cell lysate
      Lane 3 : A431 cell lysate
      Lane 4 : 293T cell lysate
      Lane 5 : K562 cell lysate
      Lane 6 : A549 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 30 kDa
      Observed band size: 26 kDa (why is the actual band size different from the predicted?)

    文献

    This product has been referenced in:
    • Boutoual R  et al. Defects in the mitochondrial-tRNA modification enzymes MTO1 and GTPBP3 promote different metabolic reprogramming through a HIF-PPAR?-UCP2-AMPK axis. Sci Rep 8:1163 (2018). Read more (PubMed: 29348686) »
    • Chung HK  et al. Growth differentiation factor 15 is a myomitokine governing systemic energy homeostasis. J Cell Biol 216:149-165 (2017). WB . Read more (PubMed: 27986797) »

    See all 10 Publications for this product

    客户评价及客户问答

    Thank you for contacting Abcam regarding ab124822.


    Regarding the use of this antibody in ICC or IHC-P, this antibody failed to react in these applications during routine QC testing. It is possible that with further troubleshooting the ant...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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