概述

  • 产品名称Anti-CLPP抗体[EPR7133]
    参阅全部 CLPP 一抗
  • 描述
    兔单克隆抗体[EPR7133] to CLPP
  • 经测试应用适用于: WB, IP, Flow Cytmore details
    不适用于: ICC or IHC-P
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive)

  • 阳性对照
    • Fetal muscle, Saos-2, A431, 293T, K562, and A549 lysates; K562 cells
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

性能

应用

Our Abpromise guarantee covers the use of ab124822 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/10000. Detects a band of approximately 26 kDa (predicted molecular weight: 30 kDa).
IP 1/10 - 1/100.
Flow Cyt 1/10 - 1/100. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
  • 应用说明Is unsuitable for ICC or IHC-P.
  • 靶标

    • 功能Clp cleaves peptides in various proteins in a process that requires ATP hydrolysis. Clp may be responsible for a fairly general and central housekeeping function rather than for the degradation of specific substrates.
    • 组织特异性Predominantly expressed in skeletal muscle. Intermediate levels in heart, liver and pancreas. Low in brain, placenta, lung and kidney.
    • 序列相似性Belongs to the peptidase S14 family.
    • 细胞定位Mitochondrion matrix.
    • Information by UniProt
    • 数据库链接
    • 别名
      • ATP dependent protease ClpAP (E coli) proteolytic subunit antibody
      • ATP dependent protease ClpAP proteolytic subunit antibody
      • ATP dependent protease ClpAP proteolytic subunit human antibody
      • ATP dependent proteolytic subunit homolog (E coli) antibody
      • ATP dependent proteolytic subunit homolog antibody
      • Caseinolytic peptidase ATP dependent proteolytic subunit homolog antibody
      • Caseinolytic protease ATP dependent proteolytic subunit E coli antibody
      • clpP antibody
      • ClpP caseinolytic peptidase ATP dependent proteolytic subunit antibody
      • ClpP caseinolytic peptidase ATP dependent proteolytic subunit homolog antibody
      • CLPP_HUMAN antibody
      • Endopeptidase Clp antibody
      • mitochondrial antibody
      • Putative ATP dependent Clp protease proteolytic subunit mitochondrial antibody
      • Putative ATP dependent Clp protease proteolytic subunit antibody
      • Putative ATP-dependent Clp protease proteolytic subunit antibody
      see all

    Anti-CLPP antibody [EPR7133] 图像

    • All lanes : Anti-CLPP antibody [EPR7133] (ab124822) at 1/1000 dilution

      Lane 1 : Fetal muscle lysate
      Lane 2 : Saos-2 cell lysate
      Lane 3 : A431 cell lysate
      Lane 4 : 293T cell lysate
      Lane 5 : K562 cell lysate
      Lane 6 : A549 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 30 kDa
      Observed band size : 26 kDa (why is the actual band size different from the predicted?)
    • Flow cytometric analysis of permeabilized K562 cells using ab124822 at 1/10 dilution (red) or a rabbit IgG negative control (green).

    Anti-CLPP antibody [EPR7133] (ab124822)参考文献

    This product has been referenced in:
    • Kubota Y  et al. Novel Mechanisms for Heme-dependent Degradation of ALAS1 Protein as a Component of Negative Feedback Regulation of Heme Biosynthesis. J Biol Chem N/A:N/A (2016). Read more (PubMed: 27496948) »
    • Guo X  et al. VCP recruitment to mitochondria causes mitophagy impairment and neurodegeneration in models of Huntington's disease. Nat Commun 7:12646 (2016). Read more (PubMed: 27561680) »

    See all 3 Publications for this product

    Product Wall

    Thank you for contacting Abcam regarding ab124822.


    Regarding the use of this antibody in ICC or IHC-P, this antibody failed to react in these applications during routine QC testing. It is possible that with further troubleshooting the ant...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"