概述

  • 产品名称Anti-CLPP抗体
    参阅全部 CLPP 一抗
  • 描述
    小鼠单克隆抗体to CLPP
  • 经测试应用适用于: WB, Flow Cytmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant fragment: HQPSGGARGQ ATDIAIQAEE IMKLKKQLYN IYAKHTKQSL QVIESAMERD RYMSPMEAQE FGILDKVLVH PPQDGEDEPT LVQKEPVEAA PAAEPVPAST , corresponding to amino acids 178-278 of Human CLPP

性能

应用

Our Abpromise guarantee covers the use of ab56455 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 30 kDa.
Flow Cyt Use 1µg for 106 cells. ab170192-Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

靶标

  • 功能Clp cleaves peptides in various proteins in a process that requires ATP hydrolysis. Clp may be responsible for a fairly general and central housekeeping function rather than for the degradation of specific substrates.
  • 组织特异性Predominantly expressed in skeletal muscle. Intermediate levels in heart, liver and pancreas. Low in brain, placenta, lung and kidney.
  • 序列相似性Belongs to the peptidase S14 family.
  • 细胞定位Mitochondrion matrix.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ATP dependent protease ClpAP (E coli) proteolytic subunit antibody
    • ATP dependent protease ClpAP proteolytic subunit antibody
    • ATP dependent protease ClpAP proteolytic subunit human antibody
    • ATP dependent proteolytic subunit homolog (E coli) antibody
    • ATP dependent proteolytic subunit homolog antibody
    • Caseinolytic peptidase ATP dependent proteolytic subunit homolog antibody
    • Caseinolytic protease ATP dependent proteolytic subunit E coli antibody
    • clpP antibody
    • ClpP caseinolytic peptidase ATP dependent proteolytic subunit antibody
    • ClpP caseinolytic peptidase ATP dependent proteolytic subunit homolog antibody
    • CLPP_HUMAN antibody
    • Endopeptidase Clp antibody
    • mitochondrial antibody
    • Putative ATP dependent Clp protease proteolytic subunit mitochondrial antibody
    • Putative ATP dependent Clp protease proteolytic subunit antibody
    • Putative ATP-dependent Clp protease proteolytic subunit antibody
    see all

Anti-CLPP antibody 图像



  • Predicted band size : 30 kDa
    CLPP antibody (ab56455) at 1ug/lane + A-431 cell lysate at 25ug/lane.
  • Overlay histogram showing HepG2 cells stained with ab56455 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab56455, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HepG2 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Anti-CLPP antibody (ab56455)参考文献

This product has been referenced in:
  • Fischer F  et al. Identification of potential mitochondrial CLPXP protease interactors and substrates suggests its central role in energy metabolism. Sci Rep 5:18375 (2015). WB . Read more (PubMed: 26679294) »
  • Fischer F  et al. Human CLPP reverts the longevity phenotype of a fungal ClpP deletion strain. Nat Commun 4:1397 (2013). WB . Read more (PubMed: 23360988) »

See all 2 Publications for this product

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