The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: Use at a concentration of 2.5 µg/ml.
Peptide ELISA: at a dilution of 1/64000.
WB: Use at a concentration of 1 - 3 µg/ml. Detects a band of approximately 27-30 kDa (predicted molecular weight: 30 kDa).
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Clp cleaves peptides in various proteins in a process that requires ATP hydrolysis. Clp may be responsible for a fairly general and central housekeeping function rather than for the degradation of specific substrates.
Predominantly expressed in skeletal muscle. Intermediate levels in heart, liver and pancreas. Low in brain, placenta, lung and kidney.