Anti-Clostridium botulinum E Toxoid抗体[209F2] (ab40787)

概述

  • 产品名称Anti-Clostridium botulinum E Toxoid抗体[209F2]
    参阅全部 Clostridium botulinum E Toxoid 一抗
  • 描述
    小鼠单克隆抗体[209F2] to Clostridium botulinum E Toxoid
  • 特异性BoTox-E (2-17) 100%; BoTox-E 100% Does not cross react with BoTox-A, BoTox-B, BoTox-C or BoTox-F (1-16)
  • 经测试应用适用于: ICC/IF, Inhibition Assaymore details
  • 免疫原

    Synthetic peptide analogue of the amino terminal region of BoTox-E.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: None
    Constituents: Tissue culture supernatant
  • 纯度Tissue culture supernatant
  • 纯化说明Sterile filtered culture supernatant.
  • 克隆单克隆
  • 克隆编号209F2
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab40787 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF
Inhibition Assay
  • 应用说明IF: 1/50.
    This product has been found to stain Neuro2A cells treated with GT1b ganglioside prior to the addition of BoTox-E at a dilution of 1:50 using an indirect immunocytochemical staining procedure.

    Inhib: Use at an assay dependent dilution.
    This product has been found to inhibit the entry of BoTox-E to Neuro2A cells treated with GT1b ganglioside prior to the addition of the toxin.

    With this antibody, we have found that blocking with 5% goat or donkey serum significantly reduces background as compared to BSA or milk.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 相关性Clostridium botulinum E Toxoid acts by inhibiting neurotransmitter release. It binds to peripheral neuronal synapses, is internalized and moves by retrograde transport up the axon into the spinal cord where it can move between postsynaptic and presynaptic neurons. It inhibits neurotransmitter release by acting as a zinc endopeptidase. It has a non-toxic component which is necessary to maintain toxicity.
    • 细胞定位Secreted
    • 别名
      • BoNT/E antibody
      • Bontoxilysin e antibody
      • Botulinum neurotoxin type e non toxic component antibody
      • Botulinum neurotoxin type e precursor antibody
      • Botulism E toxoid antibody
      • Ent120 antibody
      see all

    Anti-Clostridium botulinum E Toxoid antibody [209F2] (ab40787)参考文献

    This product has been referenced in:
    • Shu Y  et al. Phosphorylation of SNAP-25 at Ser187 mediates enhancement of exocytosis by a phorbol ester in INS-1 cells. J Neurosci 28:21-30 (2008). Read more (PubMed: 18171919) »

    See 1 Publication for this product

    Product Wall

    There are currently no Abreviews or Questions for ab40787.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"