概述

  • 产品名称Anti-CLN6抗体
    参阅全部 CLN6 一抗
  • 描述
    兔多克隆抗体to CLN6
  • 经测试应用适用于: ELISA, IHC-P, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Sheep, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig, Zebrafish
  • 免疫原

    Synthetic peptide corresponding to a region within internal region amino acids 252-301 (RLFLDSNGLF LFSSFALTLL LVALWVAWLW NDPVLRKKYP GVIYVPEPWA) of Human CLN6 (NP_060352)

  • 阳性对照
    • 293T cell lysate

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab82786 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA Use at an assay dependent concentration.

Titre using peptide based assay: 1/1562500.

IHC-P 1/100.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 36 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

Anti-CLN6 antibody 图像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human pineal tissue labelling CLN6 with ab82786 at 1/100. A Cy3-conjugated donkey anti-rabbit IgG (1/200) was used as teh secondary antibody. Positive staining shown in the cytoplasm of cell bodies of pinealocytes. Magnification: 20X. Exposure time: 0.5 - 2.0 seconds. Left - DAPI. Middle - CLN6. Right - Merge.
  • Anti-CLN6 antibody (ab82786) at 1 µg/ml + 293T cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 36 kDa
    Observed band size : 36 kDa
    Additional bands at : 45 kDa. We are unsure as to the identity of these extra bands.

Anti-CLN6 antibody (ab82786)参考文献

ab82786 has not yet been referenced specifically in any publications.

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