antigen, corresponding to amino acids 446-553 of Human CLMN.
- Human liver lysate; Human kidney tissue.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
Concentration information loading...
Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
||1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Widely expressed at intermediate level.
Contains 1 actin-binding domain.
Contains 2 CH (calponin-homology) domains.
Information by UniProt
- 9330188N17Rik antibody
- AI428889 antibody
- AI788815 antibody
Immunocytochemistry/ Immunofluorescence - Anti-CLMN antibody (ab121373)
Immunofluorescent staining of Human cell line U-251MG shows positivity in nucleus and cytoplasm. Recommended concentration of ab121373 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLMN antibody (ab121373)
ab121373 at 1/100 dilution staining CLMN in Paraffin-embedded Human Kidney tissue by Immunohistochemistry.
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