Anti-CLK2抗体(ab65082)

概述

  • 产品名称Anti-CLK2抗体
    参阅全部 CLK2 一抗
  • 描述
    兔多克隆抗体to CLK2
  • 特异性ab65082 detects endogenous levels of total CLK2 protein.
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide derived from an internal region of human CLK2.

  • 阳性对照
    • Extracts from COLO205 cells and A549 cells.

性能

应用

Our Abpromise guarantee covers the use of ab65082 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Detects a band of approximately 60 kDa (predicted molecular weight: 60 kDa).
ELISA 1/20000.

靶标

  • 功能Phosphorylates serine- and arginine-rich (SR) proteins of the spliceosomal complex may be a constituent of a network of regulatory mechanisms that enable SR proteins to control RNA splicing. Phosphorylates serines, threonines and tyrosines.
  • 序列相似性Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. Lammer subfamily.
    Contains 1 protein kinase domain.
  • 翻译后修饰Autophosphorylates on all three types of residues.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CDC like kinase 2 antibody
    • CDC-like kinase 2 antibody
    • CLK 2 antibody
    • CLK kinase antibody
    • CLK2 antibody
    • Clk2 Scamp3 antibody
    • CLK2_HUMAN antibody
    • Dual specificity protein kinase CLK2 antibody
    • hCLK 2 antibody
    • hCLK2 antibody
    • Tu52 antibody
    see all

Anti-CLK2 antibody 图像

  • All lanes : Anti-CLK2 antibody (ab65082) at 1/500 dilution

    Lane 1 : Extracts from COLO205 cells
    Lane 2 : Extracts from A549 cells
    Lane 3 : Extracts from A549 cells with immunising peptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 60 kDa
    Observed band size : 60 kDa
  • Anti-CLK2 antibody (ab65082) at 1/1000 dilution (in milk for 18 hours at 4°C) + HeLa cell nuclear lysate at 50 µg

    Secondary
    An HRP-conjugated Goat anti-rabbit IgG polyclonal at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 60 kDa
    Observed band size : 60 kDa


    Exposure time : 1 minute

    This image is courtesy of an anonymous Abreview

    See Abreview

Anti-CLK2 antibody (ab65082)参考文献

ab65082 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Human mammary cell)
Loading amount 50000 cells
Specification Human mammary cell
Gel Running Conditions Reduced Denaturing (8%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

提交于 Apr 17 2012

I am very pleased to hear you would like to accept our offer and test ab65082 and/or ab86147 in immunoprecipitation. This code will give you one free primary antibody before the expiration date. To redeem this offer, please submit an Abreview for immun...

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Thank you for your patience. None of our CLK antibodies have been tested against purified protein of other CLK family members. Cross-reactivity of, for instance, a CLK1 antibody with CLK2, 3, or 4 has not been confirmed. The best we can do is p...

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Thank you for contacting us. I am very sorry for the confusion. I performed a BLAST to find out the percentage of homology between the four proteins, and the results are: -CLK1-CLK2: 53.5% -CLK1-CLK3: 14% -CLK1-CLK4: 77% -CLK2-CL...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Colon cell line)
Loading amount 20 µg
Specification Colon cell line
Gel Running Conditions Reduced Denaturing (3-8%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Jan 03 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - nuclear (HeLa)
Loading amount 50 µg
Specification HeLa
Gel Running Conditions Reduced Denaturing (10% Tris-Bicine)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

提交于 Aug 10 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"