This antibody detects endogenous levels of total CLIP3 protein.
A synthesized peptide derived from the internal region of human CLIP3.
- A549 cells and extracts from A549 cells.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
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Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||1/500 - 1/1000. Detects a band of approximately 60 kDa (predicted molecular weight: 60 kDa).
||1/500 - 1/1000.
Functions as a cytoplasmic linker protein. Involved in TGN-endosome dynamics.
Contains 3 ANK repeats.
Contains 2 CAP-Gly domains.
Microtubule association is inhibited by the ANK repeats and the Golgi localization region (GoLD).
Cytoplasm. Golgi apparatus > Golgi stack. Localized to Golgi stacks as well as on tubulovesicular elements juxtaposed to Golgi cisternae.
Information by UniProt
- 1500005P14Rik antibody
- AI844915 antibody
- CAP GLY domain containing linker protein 3 antibody
Western blot - CLIP3 antibody (ab74239)
All lanes : Anti-CLIP3 antibody (ab74239) at 1/500 dilution
Lane 1 : Extracts from A549 cells
Lane 2 : Extracts from A549 cells plus 5µg immunizing peptide
Lysates/proteins at 5 µg per lane.
Predicted band size: 60 kDa
Observed band size: 60 kDa
Immunocytochemistry/ Immunofluorescence - CLIP3 antibody (ab74239)
Immunofluorescence analysis of A549 cells using ab74239 at a 1/500 dilution.
Left image un-treated.
Right image treated with immunizing peptide.
has not yet been referenced specifically in any publications.
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