Anti-CLIP3抗体(ab74239)

概述

  • 产品名称
  • 描述
    兔多克隆抗体to CLIP3
  • 特异性
    This antibody detects endogenous levels of total CLIP3 protein.
  • 经测试应用
    适用于: WB, ICC/IF, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse
  • 免疫原

    A synthesized peptide derived from the internal region of human CLIP3.

  • 阳性对照
    • A549 cells and extracts from A549 cells.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液
    Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab74239 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Detects a band of approximately 60 kDa (predicted molecular weight: 60 kDa).
ICC/IF 1/500 - 1/1000.
ELISA 1/40000.

靶标

  • 功能
    Functions as a cytoplasmic linker protein. Involved in TGN-endosome dynamics.
  • 序列相似性
    Contains 3 ANK repeats.
    Contains 2 CAP-Gly domains.
  • 结构域
    Microtubule association is inhibited by the ANK repeats and the Golgi localization region (GoLD).
  • 细胞定位
    Cytoplasm. Golgi apparatus > Golgi stack. Localized to Golgi stacks as well as on tubulovesicular elements juxtaposed to Golgi cisternae.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 1500005P14Rik antibody
    • AI844915 antibody
    • CAP GLY domain containing linker protein 3 antibody
    • CAP-Gly domain-containing linker protein 3 antibody
    • CLIP 170 related 59 kDa protein antibody
    • CLIP-170-related 59 kDa protein antibody
    • clip3 antibody
    • CLIP3_HUMAN antibody
    • CLIPR 59 antibody
    • CLIPR-59 antibody
    • CLIPR59 antibody
    • Cytoplasmic linker protein 170 related 59 kDa protein antibody
    • Cytoplasmic linker protein 170-related 59 kDa protein antibody
    • Restin like 1 antibody
    • RSNL1 antibody
    see all

图片

  • All lanes : Anti-CLIP3 antibody (ab74239) at 1/500 dilution

    Lane 1 : Extracts from A549 cells
    Lane 2 : Extracts from A549 cells plus 5µg immunizing peptide

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 60 kDa
    Observed band size : 60 kDa
  • Immunofluorescence analysis of A549 cells using ab74239 at a 1/500 dilution.
    Left image un-treated.
    Right image treated with immunizing peptide.

文献

ab74239 has not yet been referenced specifically in any publications.

客户评价及客户问答

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Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (Primary Neuron)
Permeabilization
Yes - 0.1% Troton X-100
Specification
Primary Neuron
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
Fixative
Paraformaldehyde
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提交于 Apr 11 2017

Abreviews
Application
Western blot
Sample
Mouse Tissue lysate - whole (Kidney)
Gel Running Conditions
Non-reduced Non-Denaturing (Native)
Loading amount
30 µg
Specification
Kidney
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
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Abcam user community

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提交于 Jun 02 2016

Application
Western blot
Sample
Monkey Cell lysate - whole cell (Kidney)
Gel Running Conditions
Non-reduced Non-Denaturing (Native)
Loading amount
30 µg
Specification
Kidney
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Jun 02 2016

Application
Immunocytochemistry/ Immunofluorescence
Sample
Monkey Cell (Kidney)
Permeabilization
Yes - 0.1% triton x-100
Specification
Kidney
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 May 18 2016

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Brain)
Permeabilization
Yes - 0.3% triton x-100
Specification
Brain
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 May 18 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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