概述

  • 产品名称
  • 描述
    兔多克隆抗体to CLIC5
  • 经测试应用
    适用于: WB, ELISA, IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant human CLIC5 protein

  • 阳性对照
    • K562 cell line lysate. Human colon carcinoma tissue.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液
    Preservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • 纯度
    Ammonium Sulphate Precipitation
  • 纯化说明
    ab75948 is prepared by Saturated Ammonium Sulfate precipitation followed by dialysis against PBS.
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab75948 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/50 - 1/100. Predicted molecular weight: 47 kDa.
ELISA 1/1000.
IHC-P 1/10 - 1/50.

靶标

  • 功能
    Can insert into membranes and form poorly selective ion channels that may also transport chloride ions. May play a role in the regulation of transepithelial ion absorption and secretion. Required for normal formation of stereocilia in the inner ear and normal development of the organ of Corti (By similarity). Is required for the development and/or maintenance of the proper glomerular endothelial cell and podocyte architecture.
  • 组织特异性
    Isoform 1: Expressed in renal glomeruli endothelial cells and podocytes (at protein level).
  • 序列相似性
    Belongs to the chloride channel CLIC family.
    Contains 1 GST C-terminal domain.
  • 结构域
    Members of this family may change from a globular, soluble state to a state where the N-terminal domain is inserted into the membrane and functions as chloride channel. A conformation change of the N-terminal domain is thought to expose hydrophobic surfaces that trigger membrane insertion.
  • 细胞定位
    Cytoplasm > cell cortex. Cytoplasm > cytoskeleton. Membrane. Associates with the cortical actin cytoskeleton. Exists both as soluble cytoplasmic protein and as membrane protein with probably a single transmembrane domain and Golgi apparatus. Cytoplasm > cytoskeleton > centrosome. Membrane. Colocalized with AKAP9 at the Golgi apparatus as well as, to a lesser extent, the centrosome. Exists both as soluble cytoplasmic protein and as membrane protein with probably a single transmembrane domain (By similarity). Colocalized with podocalyxin in renal glomeruli.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Chloride intracellular channel 5 antibody
    • Chloride intracellular channel 5A antibody
    • Chloride intracellular channel protein 5 antibody
    • CLIC 5 antibody
    • CLIC 5B antibody
    • CLIC5 antibody
    • CLIC5_HUMAN antibody
    • CLIC5B antibody
    • dJ447E21.4 antibody
    • FLJ90663 antibody
    • MST 130 antibody
    • MST130 antibody
    • MSTP 130 antibody
    • MSTP130 antibody
    see all

图片

  • Anti-CLIC5 antibody (ab75948) at 1/60 dilution + K562 cell line lysate at 35 µg

    Predicted band size : 47 kDa
    Observed band size : 55 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 30 kDa. We are unsure as to the identity of these extra bands.
  • ab75948, at 1/10 dilution, staining CLIC5 in formalin-fixed, paraffin-embedded human colon carcinoma tissue by Immunohistochemistry. ab75948 was peroxidase conjugated to the secondary antibody, followed by DAB staining.

文献

ab75948 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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