Anti-CLIC4抗体(ab77626)

概述

  • 产品名称Anti-CLIC4抗体
    参阅全部 CLIC4 一抗
  • 描述
    山羊多克隆抗体to CLIC4
  • 经测试应用适用于: WB, ELISA, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat, Cow, Dog
  • 免疫原

    Synthetic peptide:

    NGLKEEDKEPLIE-C

    , corresponding to N terminal amino acids 8-20 of Human CLIC4 (NP_039234.1).

  • 阳性对照
    • Human kidney and human placenta lysates. Min6 cells transiently expressing mouse CLIC4.

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab77626 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
ELISA
ICC/IF
  • 应用说明Peptide ELISA: Antibody detection limit dilution 1/64000.
    ICC/IF: Use at a concentration of 5 µg/ml with overnight incubation.
    WB: Use at a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 29 kDa (predicted molecular weight: 29 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Can insert into membranes and form poorly selective ion channels that may also transport chloride ions. Channel activity depends on the pH. Membrane insertion seems to be redox-regulated and may occur only under oxydizing conditions. Promotes cell-surface expression of HRH3. Has alternate cellular functions like a potential role in angiogenesis or in maintaining apical-basolateral membrane polarity during mitosis and cytokinesis. Could also promote endothelial cell proliferation and regulate endothelial morphogenesis (tubulogenesis).
    • 组织特异性Detected in epithelial cells from colon, esophagus and kidney (at protein level). Expression is prominent in heart, kidney, placenta and skeletal muscle.
    • 序列相似性Belongs to the chloride channel CLIC family.
      Contains 1 GST C-terminal domain.
    • 结构域Members of this family may change from a globular, soluble state to a state where the N-terminal domain is inserted into the membrane and functions as chloride channel. A conformation change of the N-terminal domain is thought to expose hydrophobic surfaces that trigger membrane insertion.
    • 细胞定位Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasmic vesicle membrane. Nucleus matrix. Cell membrane. Mitochondrion. Cell junction. Colocalized with AKAP9 at the centrosome and midbody. Exists both as soluble cytoplasmic protein and as membrane protein with probably a single transmembrane domain. Present in an intracellular vesicular compartment that likely represent trans-Golgi network vesicles.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Chloride intracellular channel 4 antibody
      • Chloride intracellular channel 4 (mitochondrial) antibody
      • Chloride intracellular channel 4 like antibody
      • Chloride intracellular channel protein 4 antibody
      • Clic4 antibody
      • CLIC4_HUMAN antibody
      • CLIC4L antibody
      • DKFZP566G223 antibody
      • FLJ38640 antibody
      • H1 antibody
      • HUH1 antibody
      • Intracellular chloride ion channel protein p64H1 antibody
      • MC3S5 antibody
      • mtCLIC antibody
      • p64H1 antibody
      see all

    Anti-CLIC4 antibody 图像

    • Anti-CLIC4 antibody (ab77626) at 0.1 µg/ml + human kidney lysate in RIPA buffer at 35 µg

      Predicted band size : 29 kDa
      Observed band size : 29 kDa
    • Immunofluorescent staining of min6 cells transiently expressing mouse CLIC4 using ab77626 at 5µg/ml with overnight incubation. Nuclear counter staining by Hoechst.

    Anti-CLIC4 antibody (ab77626)参考文献

    ab77626 has not yet been referenced specifically in any publications.

    Product Wall

    I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement for one vial of ab76593.

    To check the status of the order please contact ou...

    Read More

    DISCOUNT CODE: Expiration date: I am very pleased to hear you would like to accept our offer and test ab77626 inWB on rat. This code will give you 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer, please submit an Abreview...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"