• 产品名称Anti-CLIC4抗体
    参阅全部 CLIC4 一抗
  • 描述
    兔多克隆抗体to CLIC4
  • 经测试应用适用于: WB, ELISA, IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant human CLIC4 protein

  • 阳性对照
    • CLIC4 transfected 293 cell lysates; human breast carcinoma tissue



Our Abpromise guarantee covers the use of ab76593 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/50 - 1/100. Predicted molecular weight: 29 kDa.
ELISA 1/1000.
IHC-P 1/10 - 1/50.


  • 功能Can insert into membranes and form poorly selective ion channels that may also transport chloride ions. Channel activity depends on the pH. Membrane insertion seems to be redox-regulated and may occur only under oxydizing conditions. Promotes cell-surface expression of HRH3. Has alternate cellular functions like a potential role in angiogenesis or in maintaining apical-basolateral membrane polarity during mitosis and cytokinesis. Could also promote endothelial cell proliferation and regulate endothelial morphogenesis (tubulogenesis).
  • 组织特异性Detected in epithelial cells from colon, esophagus and kidney (at protein level). Expression is prominent in heart, kidney, placenta and skeletal muscle.
  • 序列相似性Belongs to the chloride channel CLIC family.
    Contains 1 GST C-terminal domain.
  • 结构域Members of this family may change from a globular, soluble state to a state where the N-terminal domain is inserted into the membrane and functions as chloride channel. A conformation change of the N-terminal domain is thought to expose hydrophobic surfaces that trigger membrane insertion.
  • 细胞定位Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasmic vesicle membrane. Nucleus matrix. Cell membrane. Mitochondrion. Cell junction. Colocalized with AKAP9 at the centrosome and midbody. Exists both as soluble cytoplasmic protein and as membrane protein with probably a single transmembrane domain. Present in an intracellular vesicular compartment that likely represent trans-Golgi network vesicles.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Chloride intracellular channel 4 antibody
    • Chloride intracellular channel 4 (mitochondrial) antibody
    • Chloride intracellular channel 4 like antibody
    • Chloride intracellular channel protein 4 antibody
    • Clic4 antibody
    • CLIC4_HUMAN antibody
    • CLIC4L antibody
    • DKFZP566G223 antibody
    • FLJ38640 antibody
    • H1 antibody
    • HUH1 antibody
    • Intracellular chloride ion channel protein p64H1 antibody
    • MC3S5 antibody
    • mtCLIC antibody
    • p64H1 antibody
    see all

Anti-CLIC4 antibody 图像

  • All lanes : Anti-CLIC4 antibody (ab76593) at 1/50 dilution

    Lane 1 : Non transfected 293 cell lysates
    Lane 2 : CLIC4 transfected 293 cell lysates

    Lysates/proteins at 2 µg per lane.

    Predicted band size : 29 kDa
    Observed band size : 29 kDa
  • Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with ab76593 at a 1/10 dilution, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

Anti-CLIC4 antibody (ab76593)参考文献

ab76593 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing
Sample Rat Cell lysate - whole cell (neonatal cardiomyocytes)
Specification neonatal cardiomyocytes
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C

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提交于 Feb 24 2014