Anti-Cleaved PARP抗体(ab72805)

概述

  • 产品名称Anti-Cleaved PARP抗体
    参阅全部 Cleaved PARP 一抗
  • 描述
    兔多克隆抗体to Cleaved PARP
  • 特异性Detects endogenous levels of fragment of activated PARP resulting from cleavage adjacent to Asp214.
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse
  • 免疫原

    Synthetic peptide derived from human cleaved PARP

  • 阳性对照
    • extracts from A549 cells, treated with etoposide (25uM, 24hours)

性能

应用

Our Abpromise guarantee covers the use of ab72805 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Detects a band of approximately 25 kDa (predicted molecular weight: 24 kDa).
ELISA 1/20000.

靶标

  • 功能Involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks. Mediates the poly(ADP-ribosyl)ation of APLF and CHFR. Positively regulates the transcription of MTUS1 and negatively regulates the transcription of MTUS2/TIP150. With EEF1A1 and TXK, forms a complex that acts as a T-helper 1 (Th1) cell-specific transcription factor and binds the promoter of IFN-gamma to directly regulate its transcription, and is thus involved importantly in Th1 cytokine production. Required for PARP9 and DTX3L recruitment to DNA damage sites. PARP1-dependent PARP9-DTX3L-mediated ubiquitination promotes the rapid and specific recruitment of 53BP1/TP53BP1, UIMC1/RAP80, and BRCA1 to DNA damage sites.
  • 序列相似性Contains 1 BRCT domain.
    Contains 1 PARP alpha-helical domain.
    Contains 1 PARP catalytic domain.
    Contains 2 PARP-type zinc fingers.
  • 翻译后修饰Phosphorylated by PRKDC and TXK.
    Poly-ADP-ribosylated by PARP2. Poly-ADP-ribosylation mediates the recruitment of CHD1L to DNA damage sites.
    S-nitrosylated, leading to inhibit transcription regulation activity.
  • 细胞定位Nucleus. Nucleus, nucleolus. Localizes at sites of DNA damage.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ADP-ribosyltransferase diphtheria toxin-like 1 antibody
    • ADPRT 1 antibody
    • ADPRT antibody
    • ADPRT1 antibody
    • APOPAIN antibody
    • ARTD1 antibody
    • NAD(+) ADP-ribosyltransferase 1 antibody
    • PARP antibody
    • PARP-1 antibody
    • PARP1 antibody
    • PARP1_HUMAN antibody
    • Poly [ADP-ribose] polymerase 1 antibody
    • Poly ADP ribose polymerase 1 antibody
    • Poly[ADP-ribose] synthase 1 antibody
    • PPOL antibody
    • SCA1 antibody
    see all

Anti-Cleaved PARP antibody 图像

  • All lanes : Anti-Cleaved PARP antibody (ab72805) at 2 µg/ml

    Lane 1 : Colon cancer lysate (human)
    Lane 2 : U2OS lysate (human)
    Lane 3 : Renal Cancer Cell Line (Human)

    Lysates/proteins at 20 µg per lane.

    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 24 kDa
    Observed band size : 25 kDa (why is the actual band size different from the predicted?)

    This image is courtesy of an Abreview submitted by Antibody Solutions

    See Abreview

  • All lanes : Anti-Cleaved PARP antibody (ab72805) at 1/1000 dilution

    Lane 1 : A549 cell extract
    Lane 2 : A549 cell extract with cleaved peptide at 5 µg

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 24 kDa
    Observed band size : 24 kDa
    Additional bands at : 70 kDa. We are unsure as to the identity of these extra bands.

Anti-Cleaved PARP antibody (ab72805)参考文献

ab72805 has not yet been referenced specifically in any publications.

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Colon cancer (tissue lysate), U2OS (cell lysate) a)
Loading amount 20 µg
Specification Colon cancer (tissue lysate), U2OS (cell lysate) a
Gel Running Conditions Reduced Denaturing (Pierce precise protein gel 4-20%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Mr. Antibody Solutions

Verified customer

提交于 Apr 06 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"