Anti-Cleaved PARP抗体(ab72805)


  • 产品名称Anti-Cleaved PARP抗体
    参阅全部 Cleaved PARP 一抗
  • 描述
    兔多克隆抗体to Cleaved PARP
  • 特异性Detects endogenous levels of fragment of activated PARP resulting from cleavage adjacent to Asp214.
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse
  • 免疫原

    Synthetic peptide derived from human cleaved PARP

  • 阳性对照
    • extracts from A549 cells, treated with etoposide (25uM, 24hours)



Our Abpromise guarantee covers the use of ab72805 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Detects a band of approximately 25 kDa (predicted molecular weight: 24 kDa).
ELISA 1/20000.


  • 功能Involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks. Mediates the poly(ADP-ribosyl)ation of APLF and CHFR. Positively regulates the transcription of MTUS1 and negatively regulates the transcription of MTUS2/TIP150. With EEF1A1 and TXK, forms a complex that acts as a T-helper 1 (Th1) cell-specific transcription factor and binds the promoter of IFN-gamma to directly regulate its transcription, and is thus involved importantly in Th1 cytokine production. Required for PARP9 and DTX3L recruitment to DNA damage sites. PARP1-dependent PARP9-DTX3L-mediated ubiquitination promotes the rapid and specific recruitment of 53BP1/TP53BP1, UIMC1/RAP80, and BRCA1 to DNA damage sites.
  • 序列相似性Contains 1 BRCT domain.
    Contains 1 PARP alpha-helical domain.
    Contains 1 PARP catalytic domain.
    Contains 2 PARP-type zinc fingers.
  • 翻译后修饰Phosphorylated by PRKDC and TXK.
    Poly-ADP-ribosylated by PARP2. Poly-ADP-ribosylation mediates the recruitment of CHD1L to DNA damage sites.
    S-nitrosylated, leading to inhibit transcription regulation activity.
  • 细胞定位Nucleus. Nucleus, nucleolus. Localizes at sites of DNA damage.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ADP-ribosyltransferase diphtheria toxin-like 1 antibody
    • ADPRT 1 antibody
    • ADPRT antibody
    • ADPRT1 antibody
    • APOPAIN antibody
    • ARTD1 antibody
    • NAD(+) ADP-ribosyltransferase 1 antibody
    • PARP antibody
    • PARP-1 antibody
    • PARP1 antibody
    • PARP1_HUMAN antibody
    • Poly [ADP-ribose] polymerase 1 antibody
    • Poly ADP ribose polymerase 1 antibody
    • Poly[ADP-ribose] synthase 1 antibody
    • PPOL antibody
    • SCA1 antibody
    see all

Anti-Cleaved PARP antibody 图像

  • All lanes : Anti-Cleaved PARP antibody (ab72805) at 2 µg/ml

    Lane 1 : Colon cancer lysate (human)
    Lane 2 : U2OS lysate (human)
    Lane 3 : Renal Cancer Cell Line (Human)

    Lysates/proteins at 20 µg per lane.

    developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 24 kDa
    Observed band size : 25 kDa (why is the actual band size different from the predicted?)

    This image is courtesy of an Abreview submitted by Antibody Solutions

    See Abreview

  • All lanes : Anti-Cleaved PARP antibody (ab72805) at 1/1000 dilution

    Lane 1 : A549 cell extract
    Lane 2 : A549 cell extract with cleaved peptide at 5 µg

    Lysates/proteins at 5 µg per lane.

    Predicted band size : 24 kDa
    Observed band size : 24 kDa
    Additional bands at : 70 kDa. We are unsure as to the identity of these extra bands.

Anti-Cleaved PARP antibody (ab72805)参考文献

ab72805 has not yet been referenced specifically in any publications.

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Colon cancer (tissue lysate), U2OS (cell lysate) a)
Loading amount 20 µg
Specification Colon cancer (tissue lysate), U2OS (cell lysate) a
Gel Running Conditions Reduced Denaturing (Pierce precise protein gel 4-20%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C

Mr. Antibody Solutions

Verified customer

提交于 Apr 06 2011