概述

  • 产品名称Anti-Cleaved PARP抗体
    参阅全部 Cleaved PARP 一抗
  • 描述
    兔多克隆抗体to Cleaved PARP
  • 特异性This antibody specifically recognizes the 85 kDa fragment of cleaved PARP and can be used as marker for detecting apoptotic cells. Cleavage Site Specific Antibody, Unconjugated. The antiserum was produced against a chemically synthesized peptide corresponding to the N-terminus of cleavage site (214/215) of human PARP and will recognize Asp 214 and Gly 215.
  • 经测试应用适用于: ICC, WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide (Human) corresponding to N-terminus of cleavage site (214/215) of human PARP.

  • 阳性对照
    • HeLa cells treated with staurosporine at 0.5 µM for 5 hours.

性能

应用

Our Abpromise guarantee covers the use of ab4830 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC 1/100.
WB 1/1000. Detects a band of approximately 85 kDa (predicted molecular weight: 85 kDa).

靶标

  • 功能Involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks. Mediates the poly(ADP-ribosyl)ation of APLF and CHFR. Positively regulates the transcription of MTUS1 and negatively regulates the transcription of MTUS2/TIP150. With EEF1A1 and TXK, forms a complex that acts as a T-helper 1 (Th1) cell-specific transcription factor and binds the promoter of IFN-gamma to directly regulate its transcription, and is thus involved importantly in Th1 cytokine production. Required for PARP9 and DTX3L recruitment to DNA damage sites. PARP1-dependent PARP9-DTX3L-mediated ubiquitination promotes the rapid and specific recruitment of 53BP1/TP53BP1, UIMC1/RAP80, and BRCA1 to DNA damage sites.
  • 序列相似性Contains 1 BRCT domain.
    Contains 1 PARP alpha-helical domain.
    Contains 1 PARP catalytic domain.
    Contains 2 PARP-type zinc fingers.
  • 翻译后修饰Phosphorylated by PRKDC and TXK.
    Poly-ADP-ribosylated by PARP2. Poly-ADP-ribosylation mediates the recruitment of CHD1L to DNA damage sites.
    S-nitrosylated, leading to inhibit transcription regulation activity.
  • 细胞定位Nucleus. Nucleus, nucleolus. Localizes at sites of DNA damage.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ADP-ribosyltransferase diphtheria toxin-like 1 antibody
    • ADPRT 1 antibody
    • ADPRT antibody
    • ADPRT1 antibody
    • APOPAIN antibody
    • ARTD1 antibody
    • NAD(+) ADP-ribosyltransferase 1 antibody
    • PARP antibody
    • PARP-1 antibody
    • PARP1 antibody
    • PARP1_HUMAN antibody
    • Poly [ADP-ribose] polymerase 1 antibody
    • Poly ADP ribose polymerase 1 antibody
    • Poly[ADP-ribose] synthase 1 antibody
    • PPOL antibody
    • SCA1 antibody
    see all

Anti-Cleaved PARP antibody 图像

  • Proteins from cell extracts were resolved by SDS-PAGE on a 4-20% Tris glycine gel and were transferred to PVDF membrane. Membranes were incubated with either 1 µg/mL anti-PARP (pan) antibody or anti PARP cleavage site (214/215) specific antibody at 1 µg/mL. After washing, membranes were incubated with goat F(ab’)2 antirabbit IgG alkaline phosphatase and bands were detected using the Tropix WesternStar detection method. The data show that the anti-PARP cleavage site specific antibody only recognizes the 85 kDa fragment of PARP in apoptotic cells (lane 3) and does not react with full length PARP (lane 1). The PARP (pan) antibody confirms that nonapoptotic cells express full length PARP of 116 kDa (lane 2) and which is cleaved when apoptosis is induced (lane 4).
  • HeLa cells were induced into apoptosis with staurosporine at 0.5 µM for 5 hours (panel A) and were untreated as control (panel B). The cells were fixed in cold acetone for 5 minutes. Cells were incubated with the anti PARP cleavage site specific antibody (CCSA) at 10 µg/mL. Cells were then incubated with biotinylated goat anti-rabbit Igs followed by ABC and DAB. The data show that the anti-PARP CCSA specifically recognizes PARP in apoptotic cells. Taken together with Western blot data above, these data demonstrate the specificity of the anti-PARP CCSA for cleaved PARP.

  • All lanes : Anti-Cleaved PARP antibody (ab4830) at 1/1000 dilution

    Lane 1 : Non-induced Jurkat cells
    Lane 2 : Induced Jurkat cells

    Secondary
    Goat Anti-Rabbit HRP
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 85 kDa
    Observed band size : 85 kDa


    Exposure time : 5 seconds

    See Abreview

Anti-Cleaved PARP antibody (ab4830)参考文献

This product has been referenced in:
  • Ibrahim MY  et al. a-Mangostin from Cratoxylum arborescens demonstrates apoptogenesis in MCF-7 with regulation of NF-?B and Hsp70 protein modulation in vitro, and tumor reduction in vivo. Drug Des Devel Ther 8:1629-47 (2014). WB ; Human . Read more (PubMed: 25302018) »
  • Ibrahim MY  et al. Involvement of NF-?B and HSP70 signaling pathways in the apoptosis of MDA-MB-231 cells induced by a prenylated xanthone compound, a-mangostin, from Cratoxylum arborescens. Drug Des Devel Ther 8:2193-211 (2014). WB ; Human . Read more (PubMed: 25395836) »

See all 10 Publications for this product

Product Wall

Application Western blot
Loading amount 50 µg
Gel Running Conditions Reduced Denaturing (10% gel)
Sample Human Tissue lysate - whole (Vastus lateralis muscle)
Specification Vastus lateralis muscle
Blocking step Milk as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Feb 04 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Tissue lysate - whole (Skeletal muscle)
Loading amount 20 µg
Specification Skeletal muscle
Gel Running Conditions Reduced Denaturing (8% Gel)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Username

Dr. G.K Sakellariou

Verified customer

提交于 May 14 2013

Thank you for contacting Abcam with this inquiry. Unfortunately the homology between the immunogen of ab52294 and bovine Caspase-3 falls below the threshold for qualification for our Abreview testing discount. Please let me know if you have any ...

Read More

Thank you for contacting us. We have several antibodies which can be used to detect PARP in Western blotting. From the study I believe you wish to detect both the cleaved (C-terminal fragment) and the full length PARP form in mouse samples? Thi...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (glioma cells)
Loading amount 20 µg
Specification glioma cells
Treatment 0.5 mM staurosporine in DMSO for 6hrs
Gel Running Conditions Non-reduced Denaturing (10%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Jun 08 2010

I'm sorry to hear you are having a problem with ab4830. I did have a question about the samples you used. What kind of cells did you test? Were these treated to induce apoptosis? This antibody will only detect cleaved PARP, so if the cells are no...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Jurkat cells)
Specification Jurkat cells
Blocking step BSA as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5%
Username

Mr. Adam Szadkowski

Verified customer

提交于 Jan 26 2006

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"