重组
RabMAb

Anti-Cleaved alpha-fodrin (Asp1185)抗体[EPR3830-17] (ab92373)

概述

  • 产品名称
    Anti-Cleaved alpha-fodrin (Asp1185)抗体[EPR3830-17]
  • 描述
    兔单克隆抗体[EPR3830-17] to Cleaved alpha-fodrin (Asp1185)
  • 特异性
    Only detects the large fragment of Alpha Fodrin resulting from cleavage at aspartic acid 1185.
  • 经测试应用
    适用于: WB, IPmore details
    不适用于: Flow Cyt,ICC or IHC-P
  • 种属反应性
    与反应: Human
  • 免疫原

    corresponding to Human Cleaved alpha-fodrin (Asp1185) aa 1150-1250.

  • 阳性对照
    • HeLa + Camptothecin cell lysate, HeLa cell lysate, Jurkat + Staurosporine cell lysate, Jurkat cell lysate
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab92373 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/10000 - 1/50000. Predicted molecular weight: 285 kDa.
IP 1/10 - 1/100.
  • 应用说明
    Is unsuitable for Flow Cyt,ICC or IHC-P.
  • 靶标

    • 功能
      Fodrin, which seems to be involved in secretion, interacts with calmodulin in a calcium-dependent manner and is thus candidate for the calcium-dependent movement of the cytoskeleton at the membrane.
    • 疾病相关
      Epileptic encephalopathy, early infantile, 5
    • 序列相似性
      Belongs to the spectrin family.
      Contains 3 EF-hand domains.
      Contains 1 SH3 domain.
      Contains 23 spectrin repeats.
    • 翻译后修饰
      Phosphorylation of Tyr-1176 decreases sensitivity to cleavage by calpain in vitro.
    • 细胞定位
      Cytoplasm, cytoskeleton. Cytoplasm, cell cortex. Expressed along the cell membrane in podocytes and presumptive tubule cells during glomerulogenesis and is expressed along lateral cell margins in tubule cells.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Alpha-II spectrin antibody
      • Fodrin alpha chain antibody
      • non-erythrocytic 1 antibody
      • non-erythroid alpha subunit antibody
      • Spectrin alpha chain antibody
      • Spectrin antibody
      • SPTAN1 antibody
      • SPTN1_HUMAN antibody
      see all

    Anti-Cleaved alpha-fodrin (Asp1185) antibody [EPR3830-17] 图像

    • All lanes : Anti-Cleaved alpha-fodrin (Asp1185) antibody [EPR3830-17] (ab92373)

      Lane 1 : HeLa + Camptothecin cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : Jurkat + Staurosporine cell lysate
      Lane 4 : Jurkat cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 285 kDa
      Observed band size : 150 kDa (why is the actual band size different from the predicted?)

    Anti-Cleaved alpha-fodrin (Asp1185) antibody [EPR3830-17] (ab92373)参考文献

    ab92373 has not yet been referenced specifically in any publications.

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