概述

  • 产品名称Anti-CLCNKA抗体
    参阅全部 CLCNKA 一抗
  • 描述
    兔多克隆抗体to CLCNKA
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Cow, Cat, Pig
  • 免疫原

    A synthetic peptide corresponding to a region of human CLCNKA

  • 阳性对照
    • Fetal liver lysate.

性能

应用

Our Abpromise guarantee covers the use of ab75175 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 75 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

Titre using peptide based assay: 1/62500.

靶标

  • 相关性CLCNKA is a member of the CLC family of voltage-gated chloride channels which have several functions including the regulation of cell volume; membrane potential stabilization, signal transduction and transepithelial transport. It is predicted to have 12 transmembrane domains, and requires a beta subunit called barttin to form a functional channel. It is thought to function in salt reabsorption in the kidney, which may be important in urinary concentrating mechanisms, and potassium recycling in the inner ear.
  • 细胞定位Membrane; Multi-pass membrane protein.
  • 数据库链接
  • 别名
    • C75963 antibody
    • Chloride channel Ka antibody
    • Chloride channel kidney A antibody
    • Chloride channel protein ClC Ka antibody
    • Chloride channel protein ClC-Ka antibody
    • ClC K1 antibody
    • ClC-K1 antibody
    • CLCK1 antibody
    • hClC Ka antibody
    • MGC61490 antibody
    • MGC93313 antibody
    • RP23-308N2.5 antibody
    see all

Anti-CLCNKA antibody 图像

  • Anti-CLCNKA antibody (ab75175) at 1 µg/ml (in 5% skim milk / PBS buffer) + Fetal liver lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 75 kDa
    Observed band size : 70 kDa (why is the actual band size different from the predicted?)

Anti-CLCNKA antibody (ab75175)参考文献

ab75175 has not yet been referenced specifically in any publications.

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