ab75008 detects endogenous levels of total CLCN4 protein.
Synthesized peptide derived from an internal region of human CLCN4.
- WB: Extracts from MCF-7 cells
ICC/IF: HUVEC cells
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
Concentration information loading...
Immunogen affinity purified
The antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen.
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||1/500 - 1/1000. Predicted molecular weight: 85 kDa.
||1/500 - 1/1000.
Proton-coupled chloride transporter. Functions as antiport system and exchanges chloride ions against protons.
Abundant in skeletal muscle and also detectable in brain and heart.
Belongs to the chloride channel (TC 2.A.49) family. ClC-4/CLCN4 subfamily.
Contains 2 CBS domains.
Early endosome membrane. Late endosome membrane.
Information by UniProt
- Chloride channel 4 antibody
- Chloride channel protein 4 antibody
- Chloride channel, voltage sensitive 4 antibody
Western blot - CLCN4 antibody (ab75008)
All lanes : Anti-CLCN4 antibody (ab75008) at 1/500 dilution
Lane 1 : extracts from MCF-7 cells
Lane 2 : extracts from MCF-7 cells with immunising peptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size : 85 kDa
Observed band size : 85 kDa
Additional bands at : 100 kDa,45 kDa. We are unsure as to the identity of these extra bands.
Immunocytochemistry/ Immunofluorescence - CLCN4 antibody (ab75008)
Immunofluorescence analysis of CLCN4 in HUVEC cells, using ab75008 at a 1/500 dilution with (right image) or without (left image) the immunising peptide.
has not yet been referenced specifically in any publications.
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