参阅全部 CLCN4 一抗
- 特异性ab75008 detects endogenous levels of total CLCN4 protein.
WB, ELISA, ICC/IFmore details
Synthesized peptide derived from an internal region of human CLCN4.
- WB: Extracts from MCF-7 cells
ICC/IF: HUVEC cells
- 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
- 存储溶液Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
Concentration information loading...
- 纯度Immunogen affinity purified
- 纯化说明The antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen.
Our Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||1/500 - 1/1000. Predicted molecular weight: 85 kDa.
||1/500 - 1/1000.
- 功能Proton-coupled chloride transporter. Functions as antiport system and exchanges chloride ions against protons.
- 组织特异性Abundant in skeletal muscle and also detectable in brain and heart.
- 序列相似性Belongs to the chloride channel (TC 2.A.49) family. ClC-4/CLCN4 subfamily.
Contains 2 CBS domains.
- 细胞定位Early endosome membrane. Late endosome membrane.
Information by UniProt
- Chloride channel 4 antibody
- Chloride channel protein 4 antibody
- Chloride channel, voltage sensitive 4 antibody
Anti-CLCN4 antibody 图像
Western blot - CLCN4 antibody (ab75008)
All lanes : Anti-CLCN4 antibody (ab75008) at 1/500 dilution
Lane 1 : extracts from MCF-7 cells
Lane 2 : extracts from MCF-7 cells with immunising peptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size : 85 kDa
Observed band size : 85 kDa
Additional bands at : 100 kDa,45 kDa. We are unsure as to the identity of these extra bands.
Immunocytochemistry/ Immunofluorescence - CLCN4 antibody (ab75008)
Immunofluorescence analysis of CLCN4 in HUVEC cells, using ab75008 at a 1/500 dilution with (right image) or without (left image) the immunising peptide.
Anti-CLCN4 antibody (ab75008)参考文献
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"