Anti-Claudin 7 (phospho Y210)抗体(ab78198)

概述

  • 产品名称Anti-Claudin 7 (phospho Y210)抗体
    参阅全部 Claudin 7 一抗
  • 描述
    兔多克隆抗体to Claudin 7 (phospho Y210)
  • 特异性This antibody detects endogenous levels of Claudin 7 only when phosphorylated at tyrosine 210.
  • 经测试应用适用于: ELISA, WBmore details
  • 种属反应性
    与反应: Rat, Human
    预测可用于: Mouse
  • 免疫原

    Synthesized phosphopeptide derived from human Claudin 7 around the phosphorylation site of tyrosine 210 (S-K-E-YP-V).

  • 阳性对照
    • Extracts from rat liver cells.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab78198 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/10000.
WB 1/500 - 1/1000. Detects a band of approximately 30 kDa (predicted molecular weight: 23 kDa).

靶标

  • 功能Plays a major role in tight junction-specific obliteration of the intercellular space.
  • 组织特异性Expressed in kidney, lung and prostate. Isoform 1 seems to be predominant, except in some normal prostate samples, where isoform 2 is the major form. Down-regulated in breast cancers, including ductal carcinoma in situ (DCIS), lobular carcinoma in situ (LCIS) and invasive ductal carcinoma (IDC) (at protein level), as well as in several cancer cell lines. Loss of expression correlates with histological grade, occurring predominantly in high-grade lesions.
  • 序列相似性Belongs to the claudin family.
  • 翻译后修饰Phosphorylated.
  • 细胞定位Cell membrane. Lateral cell membrane. Cell junction > tight junction. Co-localizes with EPCAM at the lateral cell membrane and tight junction.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CEPTR L2 antibody
    • CEPTRL 2 antibody
    • CEPTRL2 antibody
    • Claudin 1 antibody
    • Claudin 9 antibody
    • Claudin-7 antibody
    • Claudin1 antibody
    • Claudin7 antibody
    • Claudin9 antibody
    • CLD7_HUMAN antibody
    • CLDN 7 antibody
    • CLDN-7 antibody
    • CLDN7 antibody
    • Clostridium perfringens enterotoxin receptor like 2 antibody
    • CPETR L2 antibody
    • CPETRL 2 antibody
    • CPETRL2 antibody
    • Hs.84359 antibody
    see all

Anti-Claudin 7 (phospho Y210) antibody 图像

  • All lanes : Anti-Claudin 7 (phospho Y210) antibody (ab78198) at 1/500 dilution

    Lane 1 : Extracts from rat liver cells
    Lane 2 : Extracts from rat liver cells plus 5µg immunizing peptide

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 23 kDa
    Observed band size : 30 kDa (why is the actual band size different from the predicted?)

Anti-Claudin 7 (phospho Y210) antibody (ab78198)参考文献

ab78198 has not yet been referenced specifically in any publications.

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