概述

  • 产品名称Anti-Claudin 7抗体
    参阅全部 Claudin 7 一抗
  • 描述
    兔多克隆抗体to Claudin 7
  • 经测试应用适用于: WB, ICC/IFmore details
  • 种属反应性
    与反应: Human
    预测可用于: Dog, Pig
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 100 - 200 of Human Claudin 7.

    (Peptide available as ab108461.)

  • 阳性对照
    • This antibody gave a positive signal in Human prostate tissue lysate.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

相关产品

应用

Our Abpromise guarantee covers the use of ab95221 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 20 kDa (predicted molecular weight: 22 kDa).
ICC/IF Use a concentration of 5 µg/ml.

靶标

  • 功能Plays a major role in tight junction-specific obliteration of the intercellular space.
  • 组织特异性Expressed in kidney, lung and prostate. Isoform 1 seems to be predominant, except in some normal prostate samples, where isoform 2 is the major form. Down-regulated in breast cancers, including ductal carcinoma in situ (DCIS), lobular carcinoma in situ (LCIS) and invasive ductal carcinoma (IDC) (at protein level), as well as in several cancer cell lines. Loss of expression correlates with histological grade, occurring predominantly in high-grade lesions.
  • 序列相似性Belongs to the claudin family.
  • 翻译后修饰Phosphorylated.
  • 细胞定位Cell membrane. Lateral cell membrane. Cell junction > tight junction. Co-localizes with EPCAM at the lateral cell membrane and tight junction.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CEPTR L2 antibody
    • CEPTRL 2 antibody
    • CEPTRL2 antibody
    • Claudin 1 antibody
    • Claudin 9 antibody
    • Claudin-7 antibody
    • Claudin1 antibody
    • Claudin7 antibody
    • Claudin9 antibody
    • CLD7_HUMAN antibody
    • CLDN 7 antibody
    • CLDN-7 antibody
    • CLDN7 antibody
    • Clostridium perfringens enterotoxin receptor like 2 antibody
    • CPETR L2 antibody
    • CPETRL 2 antibody
    • CPETRL2 antibody
    • Hs.84359 antibody
    see all

Anti-Claudin 7 antibody 图像

  • Anti-Claudin 7 antibody (ab95221) at 1 µg/ml + Human prostate tissue lysate (cirrhosis) - total protein (ab30307) at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 22 kDa
    Observed band size : 22 kDa
    Additional bands at : 100 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 12 minutes
  • ICC/IF image of ab95221 stained MCF7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab95221, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-Claudin 7 antibody (ab95221)参考文献

ab95221 has not yet been referenced specifically in any publications.

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