概述

  • 产品名称Anti-Claudin 7抗体
    参阅全部 Claudin 7 一抗
  • 描述
    兔多克隆抗体to Claudin 7
  • 特异性ab79481 detects endogenous levels of total Claudin 7 protein
  • 经测试应用适用于: WB, ELISA, IHC-Pmore details
  • 种属反应性
    与反应: Rat, Human
    预测可用于: Mouse
  • 免疫原

    Synthetic non phosphopeptide derived from human Claudin 7 around the phosphorylation site of tyrosine 210 (SKEYPV)

  • 阳性对照
    • IHC: human prostate carcinoma tissue WB: extracts from rat liver cells

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明ab79481 was purified by affinity chromatography using an epitope specific immunogen.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab79481 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Predicted molecular weight: 23 kDa.
ELISA 1/10000.
IHC-P 1/50 - 1/100.

靶标

  • 功能Plays a major role in tight junction-specific obliteration of the intercellular space.
  • 组织特异性Expressed in kidney, lung and prostate. Isoform 1 seems to be predominant, except in some normal prostate samples, where isoform 2 is the major form. Down-regulated in breast cancers, including ductal carcinoma in situ (DCIS), lobular carcinoma in situ (LCIS) and invasive ductal carcinoma (IDC) (at protein level), as well as in several cancer cell lines. Loss of expression correlates with histological grade, occurring predominantly in high-grade lesions.
  • 序列相似性Belongs to the claudin family.
  • 翻译后修饰Phosphorylated.
  • 细胞定位Cell membrane. Lateral cell membrane. Cell junction > tight junction. Co-localizes with EPCAM at the lateral cell membrane and tight junction.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CEPTR L2 antibody
    • CEPTRL 2 antibody
    • CEPTRL2 antibody
    • Claudin 1 antibody
    • Claudin 9 antibody
    • Claudin-7 antibody
    • Claudin1 antibody
    • Claudin7 antibody
    • Claudin9 antibody
    • CLD7_HUMAN antibody
    • CLDN 7 antibody
    • CLDN-7 antibody
    • CLDN7 antibody
    • Clostridium perfringens enterotoxin receptor like 2 antibody
    • CPETR L2 antibody
    • CPETRL 2 antibody
    • CPETRL2 antibody
    • Hs.84359 antibody
    see all

Anti-Claudin 7 antibody 图像

  • All lanes : Anti-Claudin 7 antibody (ab79481) at 1/500 dilution

    Lane 1 : extracts from rat liver cells
    Lane 2 : extracts from rat liver cells with immunizing peptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 23 kDa
    Observed band size : 31 kDa (why is the actual band size different from the predicted?)
  • Immunohistochemical analysis of Claudin 7 in paraffin embedded human prostate carcinoma tissue, using ab79481 at a 1/100 dilution in the absence (left image) and presence (right image) of the immunizing peptide.

Anti-Claudin 7 antibody (ab79481)参考文献

ab79481 has not yet been referenced specifically in any publications.

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