Anti-Claudin 6抗体(ab99226)

概述

  • 产品名称
    Anti-Claudin 6抗体
    参阅全部 Claudin 6 一抗
  • 描述
    兔多克隆抗体to Claudin 6
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 200 to the C-terminus of Human Claudin 6.

  • 阳性对照
    • This antibody gave a positive signal in the following lysates: A498 (Human Kidney Carcinoma) Whole Cell Lysate

性能

应用

Our Abpromise guarantee covers the use of ab99226 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 24 kDa (predicted molecular weight: 23 kDa).

靶标

  • 功能
    Plays a major role in tight junction-specific obliteration of the intercellular space.
  • 序列相似性
    Belongs to the claudin family.
  • 细胞定位
    Cell junction > tight junction. Cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Claudin-6 antibody
    • Claudin6 antibody
    • CLD6_HUMAN antibody
    • CLDN 6 antibody
    • CLDN6 antibody
    • OTTHUMP00000159248 antibody
    • Skullin 2 antibody
    • Skullin antibody
    • UNQ757/PRO1488 antibody
    see all

图片

  • Anti-Claudin 6 antibody (ab99226) at 1 µg/ml + A498 (Human Kidney Carcinoma) Whole Cell Lysate at 10 µg

    Secondary
    Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 23 kDa
    Observed band size : 24 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 47 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 20 minutes

实验方案

文献

ab99226 has not yet been referenced specifically in any publications.

客户评价及客户问答

Application
Western blot
Sample
Human Cell lysate - whole cell (Human CLDN6 overexpression)
Gel Running Conditions
Reduced Denaturing (4-20% gradient gel)
Loading amount
20 µg
Specification
Human CLDN6 overexpression
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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提交于 Dec 02 2016

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